The blue reaction product in horseradish peroxidase neurohistochemistry: incubation parameters and visibility.
The blue reaction product in horseradish peroxidase neurohistochemistry: incubation parameters and visibility.
复制标题
辣根过氧化物酶神经组织化学中的蓝色反应产物：孵育参数和可见性。
DOI：
10.1177/24.12.63512
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
M. Mesulam
中科院分区：
文献类型：
--
作者：
M. Mesulam
当联苯胺用作色原时，在含有辣根过氧化物酶（HRP）活性的位点形成蓝色反应产物。用中性红作为复染，这种方法提供了很好的可视化逆行和顺行轴突运输的脑内注射HRP。该蓝色反应产物的可见性优于在常用的二氨基联苯胺方法中获得的棕色反应产物的可见性。孵育时间和试剂浓度的变化导致在何种程度上运输HRP可以证明联苯胺的显着差异。这些联苯胺程序之一证明了比代表性二氨基联苯胺程序更广泛的HRP转运。黑质和蓝斑核没有显示人为沉积的蓝色反应产物。
A blue reaction product is formed at sites that contain horseradish peroxidase (HRP) activity when benzidene is used as the chromogen. With neutral red as a counter stain, this method affords excellent visualization of both retrograde and orthograde axonal transport of intracerebrally injected HRP. The visibility of this blue reaction-product is better than the visibility of the brown reaction-product obtained in the commonly used diaminobenzidene procedures. Variations in incubation times and reagent concentrations resulted in significant differences in the extent to which transported HRP could be demonstrated with benzidene. One of these benzidene procedures demonstrated a wider extent of HRP transport than a representative diaminobenzidene procedure. The substantia nigra and the nucleus locus ceruleus did not display artifactual deposition of the blue reaction-product.