POLYMERIC IMMUNOGLOBULIN-M IS SECRETED BY TRANSFECTANTS OF NONLYMPHOID CELLS IN THE ABSENCE OF IMMUNOGLOBULIN-J CHAIN
POLYMERIC IMMUNOGLOBULIN-M IS SECRETED BY TRANSFECTANTS OF NONLYMPHOID CELLS IN THE ABSENCE OF IMMUNOGLOBULIN-J CHAIN
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DOI：
10.1002/j.1460-2075.1987.tb02569.x
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发表时间：
1987-09-01
期刊：
影响因子：
11.4
通讯作者：
NEUBERGER, MS
中科院分区：
文献类型：
--
作者：
CATTANEO, A;NEUBERGER, MS
构建质粒，其中编码半抗原特异性IgM抗体的重链和轻链的基因的表达在热休克启动子的控制下。胶质瘤、嗜铬细胞瘤和其他非淋巴细胞系转染质粒后，能够加工和分泌免疫球蛋白后热诱导。胶质瘤转染子进行了详细研究，并显示出分泌聚合IgM的产量与浆细胞瘤获得的产量相似。而胶质瘤IgM与J链无关，主要由五聚体和六聚体组成。因此，J链和其他淋巴特异性蛋白质都不需要聚合IgM的组装和分泌，尽管J链的缺失可能促进六聚体的形成。
Plasmids were constructed in which expression of genes encoding the heavy and light chains of a hapten-specific IgM antibody is under control of a heat shock promoter. Glioma, phaeochromocytoma and other non-lymphoid cell lines transfected with the plasmids were able to process and secrete immunoglobulin following heat induction. The glioma transfectants were studied in detail and were shown to secrete polymeric IgM in a yield similar to that obtained with a plasmacytoma. However, the glioma IgM was not associated with J chain and was largely composed of pentamers and hexamers. Thus, neither J chain nor other lymphoid-specific proteins are required for assembly and secretion of polymeric IgM although the absence of J chain may encourage hexamer formation.