Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods
Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods
复制标题
DOI：
10.1590/s0074-02762009000400007
复制
发表时间：
2009-07-01
期刊：
影响因子：
--
通讯作者：
Barardi, CRM
中科院分区：
文献类型：
--
作者：
Schlindwein, AD;Simões, CMO;Barardi, CRM
本研究的目的是比较两种核酸提取方法回收肠道病毒的活性污泥。试验样品接种人腺病毒（AdV）、甲型肝炎病毒（HAV）、脊髓灰质炎病毒（PV）和轮状病毒（RV），然后通过吸附-洗脱-沉淀法处理。使用两种提取方法：基于有机溶剂的方法和二氧化硅方法。基于有机物的方法能够从接种样品中回收20%的AdV、90%的RV以及100%的PV和HAV。二氧化硅法能够回收1.8%的AdV和90%的RV。这些结果表明，基于有机的方法更适合于检测污水污泥中的病毒。
The aim of this study was to compare two nucleic acid extraction methods for the recovery of enteric viruses from activated sludge. Test samples were inoculated with human adenovirus (AdV), hepatitis A virus (HAV), poliovirus (PV) and rotavirus (RV) and were then processed by an adsorption-elution-precipitation method. Two extraction methods were used: an organic solvent-based method and a silica method. The organic-based method was able to recoup 20% of the AdV, 90% of the RV and 100% of both the PV and HAV from seeded samples. The silica method was able to recoup 1.8% of the AdV and 90% of the RV. These results indicate that the organic-based method is more suitable for detecting viruses in sewage sludge.