Adherence-inhibitory intestinal immunoglobulin a antibody response in baboons elicited by use of a synthetic intranasal lectin-based amebiasis subunit vaccine.
Adherence-inhibitory intestinal immunoglobulin a antibody response in baboons elicited by use of a synthetic intranasal lectin-based amebiasis subunit vaccine.
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DOI：
10.1128/iai.00341-07
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发表时间：
2007-08-01
影响因子：
3.1
通讯作者：
Ravdin, Jonathan I
中科院分区：
文献类型：
--
作者：
Abd Alla, Mohamed D;White, Gary L;Ravdin, Jonathan I
我们设计了一种阿米巴病亚单位疫苗，该疫苗是通过使用四个半乳糖凝集素重亚单位的肽表位构建的，这些肽表位被来自免疫人类受试者的肠道分泌型免疫球蛋白A（伊加）抗体识别。这些表位包含在包含凝集素重亚基（命名为LC 3）的氨基酸758至1134的区域中。狒狒（Papio anubis）是溶组织内阿米巴的天然宿主;圈养的自然感染狒狒具有与人类相同的四个LC 3表位的血清伊加抗体。未感染的血清阴性狒狒每隔7天接受4次鼻内免疫接种，其中使用霍乱毒素（20 μ g）作为佐剂的合成肽疫苗（400、800或1，600 μ g/ml）。如通过酶联免疫吸附测定（ELISA）所测定的，到第28天，即最后一次免疫后7天，每剂肽疫苗在所有6只免疫狒狒中引发抗肽血清伊加和IgG以及肠伊加抗体应答（P，
We designed an amebiasis subunit vaccine that is constructed by using four peptide epitopes of the galactose-inhibitable lectin heavy subunit that were recognized by intestinal secretory immunoglobulin A (IgA) antibodies from immune human subjects. These epitopes are contained in the region encompassing amino acids 758 to 1134 of the lectin heavy subunit, designated LC3. Baboons (Papio anubis) are natural hosts for Entamoeba histolytica; naturally infected baboons raised in captivity possess serum IgA antibodies to the same four LC3 epitopes as humans. Uninfected, seronegative baboons received four intranasal immunizations at 7-day intervals with the synthetic peptide vaccine (400, 800, or 1,600 mug per nostril) with cholera toxin (20 mug) as the adjuvant. As determined by an enzyme-linked immunosorbent assay (ELISA), each dose of the peptide vaccine elicited antipeptide serum IgA and IgG and intestinal IgA antibody responses in all six immunized baboons by day 28, 7 days after the last immunization (P,