Increased metformin dosage suppresses pro-inflammatory cytokine levels in systemic circulation and might contribute to its beneficial effects.
Increased metformin dosage suppresses pro-inflammatory cytokine levels in systemic circulation and might contribute to its beneficial effects.
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DOI：
10.1080/15321819.2020.1862861
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发表时间：
2021-05-04
影响因子：
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通讯作者：
Afranie, Bright Oppong
中科院分区：
文献类型：
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作者：
Amoani, Benjamin;Sakyi, Samuel A;Afranie, Bright Oppong
2型糖尿病（T2 DM）是一种代谢紊乱，其特征在于由于胰岛素抵抗或胰岛素不足导致的血糖持续升高。二甲双胍是治疗T2 DM的推荐首选药物，已知可改善胰岛素敏感性，并通过减少慢性炎症来预防高血糖症。辅助性T细胞1型（Th 1）和17型（Th 17）是重要的促炎性CD 4 + T细胞亚群，分泌TNF-α、INF-γ（Th 1）和白细胞介素17（Th 17）。这些细胞因子已被证明在炎症、胰岛素抵抗和T2 DM的发展中起关键作用。在这里，我们探讨了不同剂量的二甲双胍对加纳T2 DM患者体循环中促炎细胞因子（TNF-α、INF-γ、GM-CSF和IL-17）水平的影响，因为炎症反应和细胞因子在二甲双胍治疗的T2 DM患者的发病机制和进展中起着重要作用。在一项基于医院的横断面研究设计中，招募了209例在加纳阿散蒂地区Komfo Anokye教学医院（KATH）糖尿病科接受治疗的知情同意T2 DM患者。采集血液样本，并通过固相夹心ELISA分析从每个参与者获得的血清中TNF-α、INF-γ、GM-CSF和IL-17细胞因子水平的浓度。我们观察到，接受3000 mg/天二甲双胍治疗的受试者TNF-α水平显著降低（p
Type 2 Diabetes Mellitus (T2DM) is a metabolic disorder, characterized by persistent elevation of blood glucose either due to insulin resistance or insulin insufficiency. Metformin is the recommended first choice of drug for the management of T2DM and is known to improve insulin sensitivity and prevents hyperglycemia by reducing chronic inflammation. T-helper type 1 (Th1) and type 17 (Th17) cells, are important pro-inflammatory CD4+ T cell subsets secreting TNF-alpha, and INF-gamma (Th1), and interleukin 17 (Th17). These cytokines have been shown to play a crucial role in inflammation, insulin resistance, and the development of T2DM. Here, we explore the effect of different metformin dosages on pro-inflammatory cytokine (TNF-alpha, INF-gamma, GM-CSF and IL-17) levels in systemic circulation among T2DM patients in Ghana, since inflammatory responses and cytokines play significant roles in the pathogenesis and progression of T2DM patients on metformin. Two hundred and nine (209) consenting T2DM patients receiving treatment at the Diabetic unit of the Komfo Anokye Teaching Hospital (KATH) in the Ashanti region of Ghana were recruited in a hospital-based cross-sectional study design. Blood samples were collected and serum obtained from each participant were analyzed for the concentrations of TNF-alpha, INF-gamma, GM-CSF and IL-17 cytokine levels by solid-phase sandwich ELISA. We observed that participants on 3000 mg/day dose of metformin had significantly lower levels of TNF-alpha (p