Host RNA polymerase II makes minimal contributions to retroviral frame-shift mutations.
Host RNA polymerase II makes minimal contributions to retroviral frame-shift mutations.
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宿主 RNA 聚合酶 II 对逆转录病毒移码突变的贡献微乎其微。
DOI：
10.1099/vir.0.80081-0
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Zhang,Jiayou
中科院分区：
文献类型：
--
作者：
Zhang,Jiayou
逆转录病毒复制过程中的变异率很高。突变可以发生在从整合的前病毒中转录病毒基因组RNA的过程中，或者在从病毒RNA反转录形成病毒DNA的过程中，或者在宿主细胞分裂时复制前病毒DNA的过程中。因此，三种聚合酶都可能参与逆转录病毒的进化：宿主RNA聚合酶II、病毒逆转录酶和宿主DNA聚合酶。由于宿主DNA聚合酶的突变率很低，突变更有可能是由宿主RNA聚合酶II和/或病毒逆转录酶引起的。建立了一种检测细胞RNA聚合酶II引起的移码突变频率以及体内单个复制周期中逆转录病毒突变率的系统。在这项研究中，确定了RNA聚合酶II对逆转录病毒复制过程中发生的移码突变的贡献率不到3% 。因此，在病毒基因组中检测到的大多数移码突变都是反转录过程中错误的结果。
The rate of mutation during retrovirus replication is high. Mutations can occur during transcription of the viral genomic RNA from the integrated provirus or during reverse transcription from viral RNA to form viral DNA or during replication of the proviral DNA as the host cell is dividing. Therefore, three polymerases may all contribute to retroviral evolution: host RNA polymerase II, viral reverse transcriptases and host DNA polymerases, respectively. Since the rate of mutation for host DNA polymerase is very low, mutations are more likely to be caused by the host RNA polymerase II and/or the viral reverse transcriptase. A system was established to detect the frequency of frame-shift mutations caused by cellular RNA polymerase II, as well as the rate of retroviral mutation during a single cycle of replicationin vivo. In this study, it was determined that RNA polymerase II contributes less than 3 % to frame-shift mutations that occur during retrovirus replication. Therefore, the majority of frame-shift mutations detected within the viral genome are the result of errors during reverse transcription.