"Turn off-on" fluorescent sensor based on quantum dots and self-assembled porphyrin for rapid detection of ochratoxin A
"Turn off-on" fluorescent sensor based on quantum dots and self-assembled porphyrin for rapid detection of ochratoxin A
复制标题
基于量子点和自组装卟啉的“关-开”荧光传感器快速检测赭曲霉毒素A
DOI：
10.1016/j.snb.2019.127212
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发表时间：
2020-01-01
影响因子：
8.4
通讯作者：
Wu, Yongjiang
中科院分区：
文献类型：
--
作者：
Liu, Li;Huang, Qingwen;Wu, Yongjiang
本文报道了一种基于ZnCdSe量子点和自组装锌卟啉的“开关型”荧光传感器，用于赭曲霉毒素A（OTA）的灵敏、快速检测。以环境友好的十二烷基二甲基甜菜碱为“软模板”，采用绿色自组装方法制备了5，10，15，20-四（4-吡啶基）-21 H-23 H-卟啉锌（ZnTPyP）纳米棒（SA-ZnTPyP）。OTA检测过程包括两个步骤：（1）ZnCdSe QD的荧光被SA-ZnTPyP显著猝灭，以及（2）SA-ZnTPyP和OTA之间的结合从它们的猝灭剂释放ZnCdSe QD，使得荧光恢复。在优化的实验条件下，该荧光传感器在0.5 ~ 80 ng mL（-1）浓度范围内具有良好的线性响应，检出限为0.33 ng mL（-1）。牛奶和咖啡样品的相对回收率在98.33%~ 103.70%之间。该荧光传感器具有良好的选择性和高灵敏度，因此可能是一个很有前途的工具，现场检测OTA在食品安全控制和环境监测。
We report herein a "turn off-on" fluorescent sensor based on ZnCdSe quantum dots (ZnCdSe QDs) and self-assembled zinc porphyrin for sensitive and rapid detection of ochratoxin A (OTA). Using environmentally friendly dodecyl dimethyl betaine as "soft template", zinc 5, 10, 15, 20-tetra(4-pyridyl)-21H-23H-porphine (ZnTPyP) was self-assembled into nanorods (SA-ZnTPyP) through a green process. The OTA detection process involves two steps: (1) the fluorescence of ZnCdSe QDs is significantly quenched by SA-ZnTPyP, and (2) the combination between SA-ZnTPyP and OTA releases ZnCdSe QDs from their quenchers such that the fluorescence is recovered. Under the optimized experimental conditions, the newly developed fluorescent sensor showed a linear response in concentration range 0.5-80 ng mL(-1), with a detection limit of 0.33 ng mL(-1). The excellent applicability of the system has been verified in milk and coffee samples, with relative recoveries between 98.33% and 103.70%. The proposed fluorescent sensor exhibits excellent selectivity and high sensitivity, and thus could be a promising tool for on-site determination of OTA in food safety control and environmental monitoring.