Identification of a novel human RAD51 homolog, RAD51B
Identification of a novel human RAD51 homolog, RAD51B
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DOI：
10.1006/geno.1997.5062
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发表时间：
1997-12-15
期刊：
影响因子：
4.4
通讯作者：
Lennon, GG
中科院分区：
文献类型：
--
作者：
Albala, JS;Thelen, MP;Lennon, GG
高度保守的酿酒酵母RAD 51蛋白在有丝分裂和减数分裂同源重组以及双链断裂修复中起作用。从公共cDNA序列数据库中筛选RAD 51样基因，从I.M.A.G.E.基因及其表达的整合分子分析（Integrated Molecular Analysis of Genes and Their Expression）一个1764 bp的cDNA克隆编码一个开放的阅读框架的350个氨基酸被分离。该克隆显示出与其他人RAD 51同源物的显著氨基酸同一性。利用一组人-仓鼠体细胞杂交和荧光原位杂交，将新的同源物命名为RAD 51 B，定位于人类染色体14 q23-q24.2。北方印迹分析表明，RAD 51 B mRNA广泛表达，并在重组活跃的组织中最丰富。与已知的RAD 51同源物相关的功能表明RAD 51 B在减数分裂重组和/或重组修复中的作用。(C)北京：科学出版社.
The highly conserved Saccharomyces cerevisiae RAD51 protein functions in both mitotic and meiotic homologous recombination and in double-strand break repair. Screening of the public cDNA sequence database for RAD51-like genes led to the identification of a partial sequence from a breast tissue library present in the I.M.A.G.E. (Integrated Molecular Analysis of Genes and their Expression) collection. An extended 1764-bp cDNA clone encoding an open reading frame of 350 amino acids was isolated. This clone showed significant amino acid identity with other human RAD51 homologs. The new homolog, named RAD51B, was mapped to human chromosome 14q23-q24.2 using a panel of human-hamster somatic cell hybrids and fluorescence in situ hybridization. Northern blot analysis demonstrated that RAD51B mRNA is widely expressed and most abundant in tissues active in recombination. Functions associated with known RAD51 homologs suggest a role for RAD51B in meiotic recombination and/or recombinational repair. (C) 1997 Academic Press.