Assessment of DNA damage in Japanese nurses handling antineoplastic drugs by the comet assay
Assessment of DNA damage in Japanese nurses handling antineoplastic drugs by the comet assay
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DOI：
10.1539/joh.50.7
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发表时间：
2008-01-01
影响因子：
3
通讯作者：
Murata, Katsuyuki
中科院分区：
文献类型：
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作者：
Sasaki, Makiko;Dakeishi, Miwako;Murata, Katsuyuki
为了阐明日本职业性暴露于抗肿瘤药物的遗传毒性效应，我们研究了日本东北部三家医院的121名女护士和46名女职员的DNA损伤，通过彗星试验进行评估。彗星试验被认为是检测单个细胞中DNA链断裂的灵敏和快速的方法，并且尾长和尾矩被用作彗星参数。在基本特征方面，46名对照组的吸烟和喝咖啡习惯率高于121名护士（P < 0.05），血红蛋白水平低于121名护士（P < 0.05）。在调整年龄和吸烟习惯等可能的协变量后，护士的对数转换尾长显著长于对照组（p < 0.05）。57名近6个月内接触过危险药物的护士的对数转换尾长显著长于46名对照组（p < 0.05），但有无接触危险药物经验的护士组的尾长和尾矩无显著性差异（p > 0.05）。这些结果表明，在医院工作过的日本护士使用抗病毒药物可能有潜在的DNA损伤风险。为了最大限度地降低日本的这一风险，除了工作人员教育和培训外，还应在医疗保健环境中使用生物安全柜和适当的防护设备。
To clarify genotoxic effects of occupational exposure to antineoplastic drugs in Japan, we examined DNA damage, assessed by the comet assay, in 121 female nurses and 46 female clerks working at three hospitals in the northeast of Japan. The comet assay is considered to be a sensitive and rapid method for DNA strand break detection in individual cells, and tail length and tail moment are used as the comet parameters. Concerning the basal characteristics, the 46 control subjects had higher rates of smoking and coffee-drinking habits and lower hemoglobin than the 121 nurses (p < 0.05). The log-transformed tail length in the nurses was significantly longer than that in the control subjects after adjusting for possible covariates such as age and smoking habit (p < 0.05). Also, the log-transformed tail length was significantly longer, in the 57 nurses who had handled antineoplastic drugs in the last six months, than that in the 46 control subjects (p < 0.05); but, no significant difference in tail length or tail moment was seen between the two nurse groups with and without experience of handling hazardous drugs (p > 0.05). These results suggest that Japanese nurses who have worked at hospitals using antineoplastic drugs may have a potential risk of DNA damage. To minimize this risk in Japan, use of biological safety cabinet and appropriate protective equipments, in addition to staff education and training, should be implemented in the healthcare environment.