Improving blood-compatibility of titanium by coating collagen-heparin multilayers
Improving blood-compatibility of titanium by coating collagen-heparin multilayers
复制标题
通过涂覆胶原蛋白-肝素多层膜改善钛的血液相容性
DOI：
10.1016/j.apsusc.2009.03.011
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发表时间：
2009-05-15
影响因子：
6.7
通讯作者：
Huang, N.
中科院分区：
文献类型：
--
作者：
Chen, J. L.;Li, Q. L.;Huang, N.
这项工作致力于改善钛的血液相容性，通过使用逐层(LBL)自组装技术在钛表面涂覆肝素(HEP)和胶原(Col)。在本文描述的工作中，LBL产生的Hep-Col薄膜的初始生长是通过在钛表面沉积一层带正电荷的聚L-赖氨酸(PLL)，该层在NaOH处理后是带负电的，然后利用静电相互作用交替沉积带负电的肝素和带正电的胶原形成多层薄膜。用傅里叶变换红外光谱、水接触角测量、扫描电子显微镜、原子力显微镜、电子分析半微量天平和XP触针轮廓仪对薄膜的化学成分、润湿性、表面形貌、质量和厚度进行了表征。采用静态血小板黏附实验，检测溶解后黏附的血小板乳酸脱氢酶(LDH)的释放，并用P-选择素测定，观察体外血小板黏附和活化情况。用活化部分凝血活酶时间(APTT)和凝血酶原时间(PT)测定凝血时间。结果表明，与未处理的钛相比，LBL膜能显著降低血小板的黏附和活化，延长APTT和PT的凝血时间。在钛表面制备的Hep-Col膜比在钛表面制备的Hep-Col膜具有更好的抗凝血性能。(C)2009年，爱思唯尔出版。
This work deals with improving the blood-compatibility of titanium by coating it with heparin (Hep) and collagen (Col) using a layer-by-layer (LBL) self-assembly technique. In the work described here, LBL-produced Hep-Col film growth is initialized by deposition of a layer of positively charged polyL-Lysine (PLL) on a titanium surface, which is negatively charged after treatment with NaOH, followed by formation of a multilayer thin film formed by alternating deposition of negatively charged heparin and positively charged collagen utilizing electrostatic interaction. The chemical composition, wettability, surface topography, mass and thickness of the film were investigated by Fourier transform infrared spectroscopy, water contact angle measurement, scanning electron microscopy, atomic force microscopy, electronic analytical semi-microbalances, and XP stylus profilometry. The in vitro platelet adhesion and activation were investigated by a static platelet adhesion test probing the lactate dehydrogenase (LDH) release of adherent platelets after lysis and by a P-selectin assay. The clotting time was examined by activated partial thromboplastin time (APTT) and prothrombin time (PT) assays. All obtained data showed that the LBL film can significantly decrease platelet adhesion and activation, and prolong clotting time of APTT and PT compared to untreated titanium. LBL-produced Hep-Col films on titanium display more excellent anticoagulation performance than on the surface of titanium. (c) 2009 Published by Elsevier B.V.