Cytopathic effects of Actinobacillus actinomycetemcomitans on monkey blood leukocytes.
Cytopathic effects of Actinobacillus actinomycetemcomitans on monkey blood leukocytes.
复制标题
伴放线放线杆菌对猴血白细胞的细胞病变作用。
DOI：
10.1111/j.1600-0765.1984.tb00802.x
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发表时间：
1984
影响因子：
3.5
通讯作者：
Hammond,BF
中科院分区：
文献类型：
--
作者：
Taichman,NS;Shenker,BJ;Tsai,CC;Glickman,LT;Baehni,PC;Stevens,R;Hammond,BF
食蟹猴（Macaca fascicularis）是否适合进行与伴放线杆菌生物学特性相关的研究是本研究的主题。我们发现正常猴子体内有白细胞毒性的A.龈下菌斑样本中的放线菌共生菌。猴外周血中性粒细胞和单核细胞在体外暴露于白细胞毒性菌株的声波提取物后被杀死。伴放线菌猴血清能抑制A.放线菌伴多糖提取物中存在中和白细胞毒素的IgG抗体。类似地，青少年牙周炎患者的血清（而不是正常人血清）消除了白细胞毒素介导的对猴PMN的杀伤。猴外周血淋巴细胞未被A杀死。放线菌共生体，但表现出抑制有丝分裂原后预孵育与A。从白细胞毒性或“非白细胞毒性”人类菌株制备的放线菌共多糖。这些研究表明，食蟹猴可作为一种合适的体外和体内模型，用于描述更多关于宿主A的信息。放线菌共生菌在人类牙周病病因学中的相互关系
The suitability of cynomolgus monkeys(Macaca fascicularis)for studies concerned with the biologic properties ofActinobacillus actinomycetemcomitansis the subject of the present investigation. We found that normal monkeys harbored leukotoxic strains ofA. actinomycetemcomitansin subgingival plaque samples. Monkey peripheral blood PMNs and monocytes were killed followingin vitroexposure to sonic extracts of leukotoxic strains ofA. actinomycetemcomitans. Monkey sera were capable of inhibiting the leukotoxic properties ofA. actinomycetemcomitanssonic extracts due to the presence of IgG antibodies which neutralized the leukotoxin. Similarly, sera from patients with juvenile periodontitis (but not normal human sera) abolished leukotoxin‐mediated killing of monkey PMNs. Monkey peripheral blood lymphoid cells were not killed byA. actinomycetemcomitansbut demonstrated depressed responses to mitogens following pre‐incubation withA. actinomycetemcomitanssonicates prepared from either leukotoxic or “non‐leukotoxic” human strains. These studies suggest that cynomolgus monkeys may serve as a suitablein vitroandin vivomodel for delineating more about host‐A. actinomycetemcomitansinterrelationships in the etiology of human periodontal disease.