Intercalation complex of proflavine with DNA: structure and dynamics by solid-state NMR.
Intercalation complex of proflavine with DNA: structure and dynamics by solid-state NMR.
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DOI：
10.1126/science.2367853
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发表时间：
1990-07
期刊：
影响因子：
56.9
通讯作者：
P. Tang;C. Juang;G. Harbison
中科院分区：
文献类型：
--
作者：
P. Tang;C. Juang;G. Harbison
用一维和二维高分辨固体核磁共振研究了插层剂前列腺素与纤维状天然DNA形成的络合物的结构。碳-13标记的前黄素被用来表明药物是以垂直于纤维轴的芳环平面堆积的，并且基本上是不动的。DNA本身的自然丰度碳-13核磁共振表明，前列腺素结合不会改变脱氧核糖环的折叠。然而，P-31核磁共振谱显示，与前列腺素结合的磷酸二酯基团的取向发生了深刻的变化，一些磷酸二酯几乎平行于螺旋轴倾斜，另一组几乎垂直。第一组磷二酸酯可能跨越了嵌入位置，而第二组的倾斜可能补偿了嵌入剂对DNA的解离。
The structure of the complex formed between the intercalating agent proflavine and fibrous native DNA was studied by one- and two-dimensional high-resolution solid-state nuclear magnetic resonance (NMR). Carbon-13-labeled proflavine was used to show that the drug is stacked with the aromatic ring plane perpendicular to the fiber axis and that it is essentially immobile. Natural abundance carbon-13 NMR of the DNA itself shows that proflavine binding does not change the puckering of the deoxyribose ring. However, phosphorus-31 NMR spectra show profound changes in the orientation of the phosphodiester grouping on proflavine binding, with some of the phosphodiesters tilting almost parallel to the helix axis, and a second set almost perpendicular. The first group to the phosphodiesters probably spans the intercalation sites, whereas the tilting of the second set likely compensates for the unwinding of the DNA by the intercalator.