Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre
Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre
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DOI：
10.1002/dvg.20310
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发表时间：
2007-06-01
期刊：
影响因子：
1.5
通讯作者：
Castrillon, Diego H.
中科院分区：
文献类型：
--
作者：
Gallardo, Teresa;Shirley, Lane;Castrillon, Diego H.
使用Cre/IoxP系统的细胞类型特异性遗传修饰是用于不同细胞谱系的遗传分析的有力工具。由于Vasa表达的精确特异性（仅限于无脊椎动物和脊椎动物物种中的生殖细胞谱系），我们假设Vasa启动子驱动的转基因Cre系将证明可用于生殖细胞谱系特异性基因失活。在这里，我们描述了一个转基因小鼠系，Vasa-Cre，Cre是有效的，特异性地表达在生殖细胞。北方分析表明，转基因表达局限于性腺。Cre介导的与Rosa 26-lacZ报告基因的重组在类似于e15时开始观察到，并且在出生时在雄性和雌性生殖细胞中的效率> 95%。虽然有一个强大的母体效应与一些动物显示更广泛的重组，有没有异位活动在大多数成年人。因此，Vasa-Cre转基因系应被证明是有用的配子发生的不同方面的遗传分析，并作为一个通用的删除线。
Cell type-specific genetic modification using the Cre/IoxP system is a powerful tool for genetic analysis of distinct cell lineages. Because of the exquisite specificity of Vasa expression (confined to the germ cell lineage in invertebrate and vertebrate species), we hypothesized that a Vasa promoter-driven transgenic Cre line would prove useful for the germ cell lineage-specific inactivation of genes. Here we describe a transgenic mouse line, Vasa-Cre, where Cre is efficiently and specifically expressed in germ cells. Northern analysis showed that transgene expression was confined to the gonads. Cre-mediated recombination with the Rosa26-lacZ reporter was observed beginning at similar to e15, and was > 95% efficient in male and female germ cells by birth. Although there was a potent maternal effect with some animals showing more widespread recombination, there was no ectopic activity in most adults. This Vasa-Cre transgenic line should thus prove useful for genetic analysis of diverse aspects of gametogenesis and as a general deletor line.