3D printed β-TCP scaffold with sphingosine 1-phosphate coating promotes osteogenesis and inhibits inflammation
3D printed β-TCP scaffold with sphingosine 1-phosphate coating promotes osteogenesis and inhibits inflammation
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DOI：
10.1016/j.bbrc.2019.03.132
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发表时间：
2019-05-14
影响因子：
3.1
通讯作者：
Ye, Qingsong
中科院分区：
文献类型：
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作者：
Cao, Yuxue;Xiao, Lan;Ye, Qingsong
同种异体骨和自体骨修复的传统治疗方法受骨替代物来源的限制。基于细胞基骨组织支架的骨组织工程是一种治疗大块骨缺损的新策略，具有生物材料可获得性、良好的生物相容性和骨传导性等优点，但炎症免疫反应仍然是影响成骨的一个问题。鞘氨醇1-磷酸(Sphingosine 1-Phate，SIP)是一种细胞来源的鞘糖脂，可介导细胞增殖、免疫调节和骨再生。我们推测，在β-磷酸三钙(β-TCP)支架上涂覆sip可以调节免疫反应并促进成骨。我们测试了涂层支架对巨噬细胞的免疫调节能力和对大鼠骨髓基质细胞的成骨能力，显示出良好的生物相容性。此外，涂层支架对炎症相关基因的表达具有剂量依赖性的抑制作用。与对照组相比，高浓度的SIP(0.5mM)可上调成骨相关基因OPN、OCN和RUNX2的表达，并显著提高碱性磷酸酶的活性。综上所述，包被蛋白的β-磷酸三钙支架具有抑制炎症、促进骨再生的作用。(C)2019 Elsevier Inc.保留所有权利。
Traditional treatments for bone repair with allografts and autografts are limited by the source of bone substitutes. Bone tissue engineering via a cell-based bone tissue scaffold is a new strategy for treatment against large bone defects with many advantages, such as the accessibility of biomaterials, good biocompatibility and osteoconductivity; however, the inflammatory immune response is still an issue that impacts osteogenesis. Sphingosine 1-phosphate (SIP) is a cell-derived sphingolipid that can mediate cell proliferation, immunoregulation and bone regeneration. We hypothesised that coating SIP on a beta-Tricalcium phosphate (beta-TCP) scaffold could regulate the immune response and increase osteogenesis. We tested the immunoregulation capability on macrophages and the osteogenic capability on rat bone marrow stromal cells of the coated scaffolds, which showed good biocompatibility. Additionally, the coated scaffolds exhibited dose-dependent inhibition of inflammatory-related gene expression. A high concentration of SIP (0.5 mu M) upregulated osteogenic-related gene expression of OPN, OCN and RUNX2, which also significantly increased the alkaline phosphatase activity, as compared with the control group. In conclusion, SIP coated beta-TCP scaffold could inhibit inflammation and promote bone regeneration. (C) 2019 Elsevier Inc. All rights reserved.