Application of Visible and Near-Infrared Reflectance Spectroscopy (Vis/NIRS) to Determine Carotenoid Contents in Banana (Musa spp.) Fruit Pulp
Application of Visible and Near-Infrared Reflectance Spectroscopy (Vis/NIRS) to Determine Carotenoid Contents in Banana (Musa spp.) Fruit Pulp
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DOI：
10.1021/jf803137d
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发表时间：
2009-03-11
影响因子：
6.1
通讯作者：
Keulemans, Johan
中科院分区：
文献类型：
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作者：
Davey, Mark W.;Saeys, Wouter;Keulemans, Johan
类胡萝卜素的分析是复杂的，这些化合物的倾向，与自由基物种反应，导致氧化分解和异构化过程中提取。因此，方案应该是快速的，并避免不必要的暴露于热、酸等。在这里，我们评估使用可见光和近红外反射光谱（维斯/NIRS）来测量从28个香蕉（香蕉和大蕉）品种的果实中的类胡萝卜素含量。首先使用标准化的RP-HPLC方案定量类胡萝卜素含量，然后将这些结果用于开发算法以从相同样品的维斯/NIR光谱预测类胡萝卜素含量。预测算法在遗传多样性品种组中的交叉验证表明，HPLC测量值与维斯/NIRS预测值之间的相关系数从总类胡萝卜素和β-胡萝卜素级分的良好（r（cv）（2），0.84，0.89）变化到α-胡萝卜素和顺式胡萝卜素的合理（r（cv）（2），0.61，0.66），但对于次要叶黄素组分只有较差的相关性（r（CV）（2），0.30）。尽管如此，由于类似于90%的芭蕉类胡萝卜素仅由α-和β-胡萝卜素组成，结果表明，维斯/近红外光谱可用于高通量筛选果肉样品的维生素A营养含量的基础上，其总类胡萝卜素含量。
The analysis of carotenoids is complicated by the tendency of these compounds to react with radical species, leading to oxidative breakdown and isomerization during extraction. Therefore, protocols should be rapid and avoid unnecessary exposure to heat, acids, and so forth. Here, we evaluate the use of visible and near infrared reflectance spectroscopy (Vis/NIRS) to measure carotenoid contents in fruit from 28 Musa (banana and plantain) varieties. Carotenoid contents were first quantified using standardized RP-HPLC protocols, and these results were then used to develop algorithms to predict carotenoid contents from Vis/NIR spectra of the same samples. Cross-validation of the predictive algorithms across a genetically diverse group of varieties demonstrated that correlation coefficients between the HPLC measurements and the Vis/NIRS predictions varied from good for the total carotenoids and beta-carotene fractions (r(cv)(2), 0.84, 0.89) to reasonable for alpha-carotene and cis-carotenes (r(cv)(2), 0.61, 0.66), but there was only a poor correlation (r(cv)(2), 0.30) for the minor lutein component. Nonetheless, since similar to 90% of the Musa carotenoids consist of only alpha- and beta-carotene, results indicate that Vis/NIRS can be used for the high-throughput screening of fruit pulp samples for vitamin A nutritional content on the basis of their total carotenoids content.