Inducible depletion of PI(4,5)P(2) by the synthetic iDePP system in Arabidopsis.
Inducible depletion of PI(4,5)P(2) by the synthetic iDePP system in Arabidopsis.
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DOI：
10.1038/s41477-021-00907-z
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发表时间：
2021-05
期刊：
影响因子：
18
通讯作者：
Caillaud MC
中科院分区：
文献类型：
--
作者：
Doumane M;Lebecq A;Colin L;Fangain A;Stevens FD;Bareille J;Hamant O;Belkhadir Y;Munnik T;Jaillais Y;Caillaud MC
磷脂酰肌醇4,5-二磷酸[PI(4,5)P2]是一种低丰度的膜脂，对质膜功能至关重要。在植物中，pi4p5激酶（PIP5K）的突变表明PI(4,5)P2的产生参与了发育、免疫和繁殖。然而，磷脂的合成是非常复杂的。因此，可能是PI(4,5)P2的稳态耗尽触发了混杂的间接效应。此外，在植物中可用的诱导工具可以增加但不降低PI(4,5)P2，并且没有可用的PIP5K抑制剂。在这里，我们介绍了iDePP (Inducible Depletion of PI(4,5)P2 in Plants)，这是一个在不到3小时内诱导和可调地耗尽植物PI(4,5)P2的系统。利用这种策略，我们证实了PI(4,5)P2对植物发育的各个方面都至关重要，包括根生长、根毛伸长和器官形成。我们发现PI(4,5)P2需要将包括AP2在内的各种内吞蛋白招募到质膜上，从而调节网格蛋白介导的内吞作用。最后，我们发现可诱导的PI(4,5)P2扰动会影响肌动蛋白细胞骨架的动力学以及微管各向异性。总之，我们提出iDePP是一种简单有效的遗传工具，可以测试PI(4,5)P2在给定细胞或发育反应中的重要性，也可以评估这种脂质在蛋白质定位中的重要性。
Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is a low abundant membrane lipids essential for plasma membrane function. In plants, mutations in PI4P 5-kinases (PIP5K) suggest that PI(4,5)P2 production is involved in development, immunity and reproduction. However, phospholipid synthesis is highly intricate. It is thus likely that steady-state depletion of PI(4,5)P2 triggers confounding indirect effects. Furthermore, inducible tools available in plants, allow to increase but not decrease PI(4,5)P2, and no PIP5K inhibitors are available. Here, we introduce iDePP (Inducible Depletion of PI(4,5)P2 in Plants), a system for the inducible and tunable depletion of PI(4,5)P2 in plants in less than three hours. Using this strategy, we confirm that PI(4,5)P2 is critical for various aspects of plant development, including root growth, root hair elongation and organ initiation. We show that PI(4,5)P2 is required to recruit various endocytic proteins, including AP2, to the plasma membrane, and thus to regulate clathrin-mediated endocytosis. Finally, we uncover that inducible PI(4,5)P2 perturbation impacts the dynamics of the actin cytoskeleton as well as microtubule anisotropy. Together, we propose that iDePP is a simple and efficient genetic tool to test the importance of PI(4,5)P2 in given cellular or developmental responses, but also to evaluate the importance of this lipid in protein localization.
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影响因子：
7.2
作者：
Gungabissoon, RA;Jiang, CJ;Hussey, PJ
通讯作者：
Hussey, PJ
影响因子：
5.5
作者：
Balla, Tamas
通讯作者：
Balla, Tamas
DOI：
10.1242/dev.155788
发表时间：
2017-10-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Gujas B;Cruz TMD;Kastanaki E;Vermeer JEM;Munnik T;Rodriguez-Villalon A
通讯作者：
Rodriguez-Villalon A
影响因子：
4.8
作者：
Huang, SJ;Blanchoin, L;Staiger, CJ
通讯作者：
Staiger, CJ
影响因子：
11.6
作者：
Ischebeck, Till;Stenzel, Irene;Heilmann, Ingo
通讯作者：
Heilmann, Ingo