Bone Regeneration by Demineralized Dentin Matrix in Skull Defects of Rats
Bone Regeneration by Demineralized Dentin Matrix in Skull Defects of Rats
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DOI：
10.2485/jhtb.21.25
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发表时间：
2012
影响因子：
0.4
通讯作者：
K. Togari;K. Miyazawa;K. Yagihashi;M. Tabuchi;H. Maeda;T. Kawai;S. Goto
中科院分区：
文献类型：
--
作者：
K. Togari;K. Miyazawa;K. Yagihashi;M. Tabuchi;H. Maeda;T. Kawai;S. Goto
牙槽骨缺损可导致功能和形态异常。在这种情况下，可以通过将新鲜的自体骨移植到缺损处来再生骨。随后，我们将重点放在第三磨牙的牙本质上，这些牙通常在拔牙后丢弃，或因正畸治疗而拔除的牙齿。与骨一样，I型胶原是牙本质中有机基质的主要成分。此外，已经证明牙本质的有机基质含有骨诱导性骨形态发生蛋白（BMP）。用拔牙牙的牙本质作为自体骨替代物进行移植是安全的。因此，本研究的目的是重复使用拔牙作为骨替代材料。将脱矿牙本质基质（DDM）移植于大鼠颅骨缺损处，观察其骨再生能力及作为骨替代材料的可能性。在本研究中，对照组的缺损边缘形成了新骨，但在移植物组中，缺损内的单个DDM颗粒形成了新骨，而不仅仅是边缘。在第8周，对照组的缺损修复有限。相反，在移植物组中，大部分缺损被骨组织覆盖。总之，DDM可能是一种有用的骨替代物，可作为骨再生的支架，在手术后不久诱导高水平的新骨形成。
Alveolar bone defects may result in functional and morphological abnormalities. In such cases, bone can be regenerated by grafting fresh autogenous bone into the defect. Subsequently, we focused on the dentin of third molars, which are usually discarded after extraction, or teeth removed faor orthodontic therapy. As in bone, type I collagen is the main component of the organic matrix in dentin. Also, it has been proven that the organic matrix of dentin contains osteoinductive bone morphogenetic protein (BMP). It would be safe to perform grafting with the dentin of extracted teeth used as an autogenous bone substitute. Therefore, the objective of the present study was to reuse extracted teeth as a bone substitute material. Demineralized dentin matrix (DDM), an organic material derived from the dentine of bovine teeth, was grafted in rat skull defects to determine its bone regeneration ability and the possibility of its use as a bone substitute. In the present study, new bone formed from the defect margin in the control group, but in the graft group, new bone formation occurred from individual DDM granules within the defect, and not just from the margin. At Week 8, defect repair was limited in the control group. In contrast, most of the defect was covered by osseous tissue in the graft group. In conclusion, DDM may be a useful bone substitute that serves as a scaffold for bone regeneration by inducing a high level of new bone formation soon after surgery.