The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice
The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice
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WRKY转录因子OsWRKY78调节水稻茎伸长和种子发育
DOI：
10.1007/s00425-011-1423-y
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发表时间：
2011-05
期刊：
影响因子：
4.3
通讯作者：
Liu Qiaoquan
中科院分区：
文献类型：
--
作者：
Zhang Changquan;Xu Yong;Lu Yan;Yu Hengxiu;Gu Minghong;Liu Qiaoquan
WRKY蛋白是一个巨大的转录调控蛋白超家族，主要参与各种植物生理程序。本研究鉴定了WRKY转录因子WRKY78在水稻中的表达谱及其可能的功能。实时RT-PCR分析显示，oswrky78转录本在伸长茎中表达量最多，但在所有器官中均有表达。通过启动子- gus分析进一步证实了该基因在转基因水稻中的表达谱。OsWRKY78::GFPfusion基因瞬时表达分析表明，OsWRKY78靶向洋葱表皮细胞核。此外，还获得了OsWRKY78RNAi转基因和过表达水稻品系。oswrky78过表达的转基因植株表现出与野生型相同的表型，而抑制oswrky78表达的转基因植株由于细胞长度减少而导致半矮化和小核表型。此外，还鉴定了一个T-DNA插入突变体lineoswrky78，并观察到与RNAi植株相似的表型。在RNAi植物中，除了胚乳淀粉晶体结构有微小变化外，籽粒品质分析数据无显著差异。综上所述，这些结果表明OsWRKY78可能是水稻茎伸长和种子发育的调节因子。
WRKY proteins are a large super family of transcriptional regulators primarily involved in various plant physiological programs. In present study, the expression profile and putative function of the WRKY transcriptional factor, WRKY78, in rice were identified. Real-time RT-PCR analysis showed thatOsWRKY78transcript was most abundant in elongating stems though its expression was detected in all the tested organs. The expression profiles were further confirmed by using promoter-GUS analysis in transgenic rice.OsWRKY78::GFPfusion gene transient expression analysis demonstrated that OsWRKY78 targeted to the nuclei of onion epidermal cell. Furthermore,OsWRKY78RNAi and overexpression transgenic rice lines were generated. Transgenic plants withOsWRKY78overexpression exhibited a phenotype identical to the wild type, whereas inhibition ofOsWRKY78expression resulted in a semi-dwarf and small kernel phenotype due to reduced cell length in transgenic plants. In addition, a T-DNA insertion mutant lineoswrky78was identified and a phenotype similar to that of RNAi plants was also observed. Grain quality analysis data showed no significant differences, with the exception of minor changes in endosperm starch crystal structure in RNAi plants. Taken together, these results suggest that OsWRKY78 may acts as a stem elongation and seed development regulator in rice.
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DOI：
10.1002/0471650129.dob0534
发表时间：
2004-10
期刊：
Dictionary of Bioinformatics and Computational Biology
影响因子：
--
作者：
M. P. Cummings
通讯作者：
M. P. Cummings
水稻种子发育和发芽过程中编码普鲁兰酶型脱支酶的 OsPUL 基因的表达特征
影响因子：
6.5
作者：
Li, Qian-Feng;Zhang, Gui-Yun;Liu, Qiao-Quan
通讯作者：
Liu, Qiao-Quan
影响因子：
4.6
作者：
J. Yazaki;Zenpei Shimatani;Akiko H. Hashimoto;Y. Nagata;F. Fujii;Keiichi Kojima;Kohji Suzuki;T. Taya;M. Tonouchi;C. Nelson;A. Nakagawa;Y. Otomo;K. Murakami;K. Matsubara;J. Kawai;Piero Carninci;Y. Hayashizaki;S. Kikuchi
通讯作者：
J. Yazaki;Zenpei Shimatani;Akiko H. Hashimoto;Y. Nagata;F. Fujii;Keiichi Kojima;Kohji Suzuki;T. Taya;M. Tonouchi;C. Nelson;A. Nakagawa;Y. Otomo;K. Murakami;K. Matsubara;J. Kawai;Piero Carninci;Y. Hayashizaki;S. Kikuchi
影响因子：
2.4
作者：
Q. Leng;Hong Yang;Qun Yang;Jianping Zhou
通讯作者：
Q. Leng;Hong Yang;Qun Yang;Jianping Zhou
DOI：
10.1073/pnas.132266399
发表时间：
2002-06-25
影响因子：
11.1
作者：
Spielmeyer, W;Ellis, MH;Chandler, PM
通讯作者：
Chandler, PM