SPECIFIC BINDING-SITES FOR INSULIN AND INSULIN-LIKE GROWTH FACTOR-I IN HUMAN ENDOMETRIAL CANCER
SPECIFIC BINDING-SITES FOR INSULIN AND INSULIN-LIKE GROWTH FACTOR-I IN HUMAN ENDOMETRIAL CANCER
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DOI：
10.1016/0002-9378(91)90047-u
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发表时间：
1991-12-01
影响因子：
9.8
通讯作者：
DOHERTY, MG
中科院分区：
文献类型：
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作者：
NAGAMANI, M;STUART, CA;DOHERTY, MG
已知胰岛素和胰岛素样生长因子I具有有丝分裂作用，因此可能在子宫内膜癌的发展中发挥作用。我们进行这项研究是为了调查人类子宫内膜癌组织是否有这些物质的受体。从10名子宫内膜癌患者的子宫切除术中获得子宫内膜癌组织样本，并从因非内分泌问题而接受子宫切除术的正常周期妇女中收集对照子宫内膜组织。碘125-胰岛素和[I-125]胰岛素样生长因子I的结合研究显示，在正常子宫内膜和子宫内膜癌组织中，胰岛素和胰岛素样生长因子I都存在特异性的结合位点。[I-125]胰岛素在子宫内膜癌组织中的结合率（平均+/- SE 2.4% +/- 0.5%/100 μ g蛋白）与正常子宫内膜的结合率（3.5% +/- 1%/100 μ g蛋白）无显著差异。相反，[I-125]胰岛素样生长因子I在子宫内膜癌中的总结合率（5.3% +/- 1.5%/100 μ g蛋白）显著高于正常子宫内膜（2.1% +/- 0.4%/100 μ g蛋白）（p < 0.04）。肿瘤的组织学分级与胰岛素样生长因子I结合呈显著正相关（r = 0.865, p < 0.02）。高亲和受体在正常子宫内膜和肿瘤子宫内膜中的亲和常数相似。这些结果提示胰岛素和胰岛素样生长因子I可能在子宫内膜癌的生长发展中发挥作用。
Insulin and insulin-like growth factor I are known to be mitogenic and therefore may play a role in the development of endometrial cancer. We undertook this study to investigate whether human endometrial cancer tissue has receptors for these substances. Endometrial cancer tissue samples were obtained at hysterectomy from 10 women with endometrial cancer, and control endometrial tissue was collected from normal cycling women undergoing hysterectomy for nonendocrine problems. Binding studies with iodine 125-insulin and [I-125]insulin-like growth factor I revealed the presence of specific binding sites for insulin and insulin-like growth factor I in both normal endometrium and endometrial cancer tissue. The percent binding of [I-125]insulin in the endometrial cancer tissue (mean +/- SE 2.4% +/- 0.5%/100-mu-g protein) was not significantly different from that in normal endometrium (3.5% +/- 1%/100-mu-g protein). On the contrary, the percent total binding of [I-125]insulin-like growth factor I in the endometrial cancer (5.3% +/- 1.5%/100-mu-g protein) was significantly (p < 0.04) higher than that observed in normal endometrium (2.1% +/- 0.4%/100-mu-g protein). There was a significant positive correlation between the histologic grade of the tumor and the insulin-like growth factor I binding (r = 0.865, p < 0.02). The affinity constants for the high-affinity receptors were similar in the normal and neoplastic endometrium. These results indicate that insulin and insulin-like growth factor I may play a role in the growth and development of endometrial cancer.