The influence of sevoflurane on the reactivity of astrocytes in the course of the experimental intracerebral haemorrhage in rat.
The influence of sevoflurane on the reactivity of astrocytes in the course of the experimental intracerebral haemorrhage in rat.
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发表时间：
2005-09
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通讯作者：
Z. Karwacki;P. Kowiański;J. Dziewiątkowski;B. Domaradzka-Pytel;B. Ludkiewicz;S. Wójcik;O. Narkiewicz;J. Morys
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作者：
Z. Karwacki;P. Kowiański;J. Dziewiątkowski;B. Domaradzka-Pytel;B. Ludkiewicz;S. Wójcik;O. Narkiewicz;J. Morys
将40只成年Wistar大鼠按麻醉方式分为两组。在两组动物中，通常用芬太尼麻醉，腹膜内给予脱氢苯并哌啶，肌肉内给予咪达唑仑。在第二组（SEVO）中，动物接受2.2 vol%潮气末浓度的七氟烷。脑内血肿是通过向纹状体中注入100微升自体血液产生的。根据存活时间的长短，每组又分为1、3、7、14、21天5个亚组。星形胶质细胞群体通过抗GFAP染色进行研究。应用体视学分析方法估计免疫反应细胞的数密度及其类型分布。观察第7天，SEVO组GFAP阳性星形胶质细胞密度低于对照组（p<0.05）。在对照组中，在第1和第3存活日之间，观察到活化星形胶质细胞百分比的增加（p<0.05），其保持在该水平直至观察结束。在SEVO组中，活化星形胶质细胞百分比在第3 ~ 7天增加（p<0.05），在第14 ~ 21天减少（p<0.05）。在观察期间，SEVO组中活化星形胶质细胞的百分比低于对照组（p<0.05）。麻醉期间给予七氟烷脑出血动物不仅引起星形胶质细胞活化延迟，而且其水平降低。
40 adult Wistar rats were divided into two groups depending on the applied anaesthesia. In both groups animals were generally anaesthetized with fentanyl, dehydrobenzperidol administered intraperitoneally and midazolam given intramuscularly. In the second group (SEVO) animals received sevoflurane of 2.2 vol% end-tidal concentration. Intracerebral haematoma was produced through infusion of 100 microl of autologous blood into the striatum. Each group was divided into five subgroups depending on the length of survival period: 1, 3, 7, 14, 21 days. The astrocytic population was studied by means of anti-GFAP staining. Stereological analysis was applied to estimate the numerical density of immunoreactive cells and the distribution of their types. On 7th day of observation the density of GFAP-immunoreactive astrocytes in SEVO was lower (p<0,05) than that in the control group. In the control group, the increase (p<0.05) of per cent of activated astrocytes between the 1st and 3rd survival day was noted, which remained at this level till the end of observation. In SEVO group, the increase (p<0.05) of per cent of activated astrocytes between the 3rd and 7th day and the decrease (p<0.05) between the 14th and 21st survival day were observed. During days of observation the per cent of activated astrocytes was lower (p<0.05) in the SEVO group than that in the control group. Administration of sevoflurane during anaesthesia to animals with intracerebral haemorrhage has evoked not only the delay of the activation of astrocytes but also decrease in its level.