THE ADHESIVE PROTEIN CDNA OF MYTILUS-GALLOPROVINCIALIS ENCODES DECAPEPTIDE REPEATS BUT NO HEXAPEPTIDE MOTIF
THE ADHESIVE PROTEIN CDNA OF MYTILUS-GALLOPROVINCIALIS ENCODES DECAPEPTIDE REPEATS BUT NO HEXAPEPTIDE MOTIF
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DOI：
10.2307/1542281
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发表时间：
1994-06-01
影响因子：
1.6
通讯作者：
ODO, S
中科院分区：
文献类型：
--
作者：
INOUE, K;ODO, S
贻贝通过它的足骨附着在坚硬的表面上，足骨由一束线组成，每条线都有一个纤维状的胶原核心，上面覆盖着粘附的蛋白质。从日本贻贝（Mytilus galloprovincialis）足部分离的RNA构建了cDNA文库。对该文库进行了与近缘种毛毛霉（M. edulis）主要粘附蛋白十肽重复基序部分对应的核苷酸序列检测，获得了包含该粘附蛋白整个编码区的克隆。尽管有一些替换和18个氨基酸的缺失，但加洛省分枝杆菌的信号区和非重复区与毛利斯分枝杆菌的序列是同源的。重复区域包括一个四肽序列和62个重复序列，与毛毛杆菌相同，但毛毛杆菌中存在的六肽序列在加洛省毛杆菌中不存在。在十肽基序中，两个酪氨酸残基、两个赖氨酸残基和两个脯氨酸残基中的一个高度保守，但其他残基经常被取代。在十肽基序的一些残基中，观察到特定的密码子用法，表明核苷酸序列本身具有功能。
A mussel is attached to hard surfaces by its byssus, which consists of a bundle of threads, each with a fibrous collagenous core coated with adhesive proteins. We constructed a cDNA library from RNA isolated from the foot of the mussel Mytilus galloprovincialis sampled in Japan. The library was probed with a nucleotide sequence corresponding to a part of the decapeptide repeat motif in the major adhesive protein of the closely related species M. edulis, and a clone including the whole coding region of the same adhesive protein of M. galloprovincialis was isolated. The sequences of the signal and nonrepetitive regions of the protein of M. galloprovincialis were homologous to those of M. edulis, despite several substitutions and a deletion of 18 amino acids. The repetitive region included a tetradecapeptide sequence and 62 repeats of the same decapeptide motif as in M. edulis, but hexapeptide sequences present in M. edulis were absent in the protein of M. galloprovincialis. In the decapeptide motif, two tyrosine residues, two lysine residues, and one of the two proline residues were highly conserved, but other residues were frequently substituted. In some residues in the decapeptide motif, specific codon usages were observed, suggesting that the nucleotide sequence itself has a function.