Increased expression of mRNA encoding interleukin (IL)-4 and its splice variant IL-4δ2 in cells from contacts of Mycobacterium tuberculosis, in the absence of in vitro stimulation
Increased expression of mRNA encoding interleukin (IL)-4 and its splice variant IL-4δ2 in cells from contacts of Mycobacterium tuberculosis, in the absence of in vitro stimulation
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DOI：
10.1111/j.1365-2567.2004.01922.x
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发表时间：
2004-08-01
期刊：
影响因子：
6.4
通讯作者：
Brookes, RH
中科院分区：
文献类型：
--
作者：
Fletcher, HA;Owiafe, P;Brookes, RH
白细胞介素（IL）-4的表达在结核病中增加，并被认为是有害的。我们在这里表明，在健康的接触，有其天然存在的拮抗剂，IL-4delta 2（IL-4delta 2）的表达增加。我们确定接触表明，他们的外周血单核细胞（PBMC）释放干扰素（IFN）-γ响应结核分枝杆菌特异性抗原6 kDa早期分泌抗原靶（ESAT-6）。来自这些接触者的新鲜未刺激PBMC含有比来自ESAT-6阴性供体（非接触者）的细胞更高水平的编码IL-4 δ 2的mRNA（P=0.002）。这些数据表明，与M.结核病引起不寻常的、以前未认识到的免疫学事件。我们初步假设进展为活动性疾病可能取决于IL-4与IL-4delta 2的潜在比率。
Expression of interleukin (IL)-4 is increased in tuberculosis and thought to be detrimental. We show here that in healthy contacts there is increased expression of its naturally occurring antagonist, IL-4delta2 (IL-4delta2). We identified contacts by showing that their peripheral blood mononuclear cells (PBMC) released interferon (IFN)-gamma in response to the Mycobacterium tuberculosis-specific antigen 6 kDa early secretory antigenic target (ESAT-6). Fresh unstimulated PBMC from these contacts contained higher levels of mRNA encoding IL-4delta2 (P=0.002) than did cells from ESAT-6 negative donors (noncontacts). These data indicate that contact with M. tuberculosis induces unusual, previously unrecognized, immunological events. We tentatively hypothesize that progression to active disease might depend upon the underlying ratio of IL-4 to IL-4delta2.