Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Muller cells of the retina
Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Muller cells of the retina
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DOI：
10.1007/s00418-010-0752-4
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发表时间：
2010-12-01
影响因子：
2.3
通讯作者：
Usuda, Nobuteru
中科院分区：
文献类型：
--
作者：
Atsuzawa, Kimie;Nakazawa, Ayami;Usuda, Nobuteru
通过免疫组织化学显示视网膜中线粒体脂肪酸β-氧化系统的存在。脂肪酸被认为是通过脂肪酸β-氧化代谢的主要能量来源，与通过糖酵解代谢的葡萄糖一起在整个身体的器官中代谢，但对视网膜中的这种代谢系统几乎一无所知。成年大鼠视网膜进行免疫荧光和免疫电子显微镜定位的脂肪酸β-氧化酶，连同蛋白质印迹分析定量的酶蛋白和基因表达的DNA微阵列分析的量。所有检测的酶都显示存在于视网膜中，但数量很少，视网膜中的蛋白质和基因表达量约为肝脏中的1/10。免疫组化在光镜和电镜水平显示的酶更优先定位于线粒体的Muller细胞比视网膜神经元。从视网膜分离Muller细胞，并确认线粒体脂肪酸β-氧化酶的存在。因此，线粒体脂肪酸β-氧化系统被证明是存在于视网膜异质性。
The presence of a mitochondrial fatty acid beta-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid beta-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid beta-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Muller cells than the retinal neurons. The Muller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid beta-oxidation enzymes. A mitochondrial fatty acid beta-oxidation system was thus shown to be present in the retina heterogeneously.