ACTIVATION AND SOMATIC MUTATION OF THE TRANSLOCATED C-MYC GENE IN BURKITT-LYMPHOMA CELLS
ACTIVATION AND SOMATIC MUTATION OF THE TRANSLOCATED C-MYC GENE IN BURKITT-LYMPHOMA CELLS
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DOI：
10.1016/0092-8674(84)90227-7
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
LEDER, P
中科院分区：
文献类型：
--
作者：
TAUB, R;MOULDING, C;LEDER, P
与 c-myc 基因及其转录物大幅扩增的其他人类肿瘤相比，对 t(8;14) Burkitt 细胞系的仔细分析表明，与对照淋巴母细胞系相比，c-myc 转录物略有增加，在某些情况下根本没有增加。相反，易位的 c-myc 基因的表达存在更微妙的变化，其特征是启动子利用率的变化以及对使这些相同细胞内正常 c-myc 等位基因失活的调节明显不敏感。在一些 Burkitt 细胞系中，这种失调可能是因为通过去除 c-myc 基因的大双启动子/前导片段而丢失了推定的控制区域。然而，在其他细胞系中，这种失调可能是由发生在推定控制区域内的体细胞突变来解释的，尽管它距离易位断点有数百个碱基。
In contrast to other human tumors in which the c-myc gene and its transcript are greatly amplified, careful analysis of t(8;14) Burkitt cell lines indicates that the c-myc transcript is marginally, and in some cases not at all, increased by comparison to control lymphoblastoid cell lines. Instead, there is a more subtle alteration in the expression of the translocated c-myc gene characterized by a shift in promoter utilization and an apparent insensitivity to the regulation that inactivates the normal c-myc allele within these same cells. In some Burkitt cell lines, such deregulation might be because of the loss of a putative control region through removal of the large dual promoter/leader segment of the c-myc gene. In other cell lines, however, this deregulation may be explained by somatic mutations that occur within the putative control region even though it is located many hundreds of bases from the translocation breakpoint.