HISTOLOGICAL AND HISTOCHEMICAL ANALYSIS OF THE FORMATION OF IMPLANTATION CHAMBERS IN THE MOUSE UTERUS

HISTOLOGICAL AND HISTOCHEMICAL ANALYSIS OF THE FORMATION OF IMPLANTATION CHAMBERS IN THE MOUSE UTERUS
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小鼠子宫着床室形成的组织学和组织化学分析

DOI：
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发表时间：
1965
期刊：
影响因子：
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通讯作者：
J. Hinchliffe
J. Hinchliffe
中科院分区：
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文献类型：
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作者：
C. Finn;J. Hinchliffe

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总结。已经发现，在大多数情况下，在假妊娠第4天向小鼠子宫注射油会导致沿着子宫长度形成几个离散的蜕膜肿胀。假孕第6天的组织化学和组织学检查显示，在肿胀的中间形成了一个类似植入腔的结构。腹膜侧的管腔向下推入肿胀的中心，腹膜侧的上皮被破坏。囊室底部周围的蜕膜组织含有糖原和促淀粉酶通过阳性物质，类似于植入囊胚周围的物质。人工植入腔腔内含有大量富含蛋白质的分泌物和抗淀淀酶的pasx=req阳性物质，可能是中性的粘多糖-蛋白复合物。这种分泌物在怀孕第6天的自然着床室中也会发现，尽管数量要少得多。这表明它是一种由子宫分泌的营养物质，作为着床反应的一部分，通常由囊胚吸收。由此可见，4天假孕小鼠子宫对自然或非自然蜕膜刺激具有反应能力，并形成着床腔。
Summary. It has been found that in the majority of cases the injection of oil into the uterus of mice on the 4th day of pseudopregnancy results in the formation of several discrete decidual swellings along the length of the uterus. Histochemical and histological examination on the 6th day of pseudopregnancy reveals that a structure closely resembling an implantation chamber is formed in the middle of the swellings. There is a down-pushing of the lumen from the mesometrial side into the centre of the swelling and breakdown of epithelium on the antimesometrial side of the chamber. The decidual tissue around the base of the chamber contains both glycogen and pas-positive diastase-fast material, similar to that found around an implanting blastocyst. The lumen of the artificial implantation chamber contains relatively large quantities of a secretion rich in protein and a diastase-fast pasx=req- positive material, probably a neutral mucopolysaccharide-protein com-plex. This secretion is also found in natural implantation chambers on the 6th day of pregnancy, though in much smaller quantities. It is suggested that it is a nutritive substance secreted by the uterus as part of the implantation reaction and normally taken up by the blastocyst. It is concluded that the 4-day pseudopregnant mouse uterus has the competence to react to a natural or unnatural decidual stimulus with the formation of implantation chambers.