Rapid succession within the Veillonella population of a developing human oral biofilm in situ
Rapid succession within the Veillonella population of a developing human oral biofilm in situ
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DOI：
10.1128/jb.01958-05
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发表时间：
2006-06-01
影响因子：
3.2
通讯作者：
Kolenbrander, Paul E.
中科院分区：
文献类型：
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作者：
Palmer, Robert J., Jr.;Diaz, Patricia I.;Kolenbrander, Paul E.
链球菌是称为龈上牙菌斑的多物种口腔生物膜的主要成分;它们通过将糖发酵成有机酸而生长，例如，乳酸。韦荣球菌是早期牙菌斑中普遍存在的一种成分，它们不能利用糖;它们将有机酸（如乳酸盐）发酵成短链酸、二氧化碳和氢的混合物。某些韦荣菌素在体外与链球菌结合（共聚集）。我们发现，4和8小时之间的斑块发展，韦荣氏球菌的优势菌株的变化，在他们的表型特征（共聚集和抗体反应性），以及在他们的基因型特征（16 S RNA基因序列以及菌株水平的指纹图谱）。这种演替与混合种细菌菌落的发展相协调。在自然生物膜的发展中，群落结构的变化可以非常迅速地发生，我们认为，这一过程可能会影响该生态系统内的进化。
Streptococci are the primary component of the multispecies oral biofilm known as supragingival dental plaque; they grow by fermentation of sugars to organic acids, e.g., lactic acid. Veillonellae, a ubiquitous component of early plaque, are unable to use sugars; they ferment organic acids, such as lactate, to a mixture of shorter-chain-length acids, CO2, and hydrogen. Certain veillonellae bind to (coaggregate with) streptococci in vitro. We show that, between 4 and 8 hours into plaque development, the dominant strains of Veillonella change in their phenotypic characteristics (coaggregation and antibody reactivity) as well as in their genotypic characteristics (16S RNA gene sequences as well as strain level fingerprint patterns). This succession is coordinated with the development of mixed-species bacterial colonies. Changes in community structure can occur very rapidly in natural biofilm development, and we suggest that this process may influence evolution within this ecosystem.