An MSN-based synergistic nanoplatform for root canal biofilm eradication via Fenton-enhanced sonodynamic therapy
An MSN-based synergistic nanoplatform for root canal biofilm eradication via Fenton-enhanced sonodynamic therapy
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基于 MSN 的协同纳米平台，通过芬顿增强声动力疗法根除根管生物膜。
DOI：
10.1039/d1tb01031j
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发表时间：
2021-07-10
影响因子：
7
通讯作者：
Huang, Cui
中科院分区：
文献类型：
--
作者：
Guo, Jingmei;Xu, Yue;Huang, Cui
灌溉剂的有效性和生物相容性对于根管治疗的成功至关重要。当前可用的灌溉剂的缺陷突出了一个事实，即在牙髓治疗中完全消毒需要更先进的技术和策略。在本研究中，为根管消毒而制造了芬顿反应增强的抗菌声动力疗法（SDT）平台。首先，将介孔二氧化硅纳米颗粒（MSN）合成，用氨基组接枝，然后与Sonosensitizer Protopoporphyrin IX（PPIX）结合。然后将铁离子锚定（m@p-fe）发起芬顿反应。按大小和ZETA电位测量值，扫描电子显微镜，透射电子显微镜和热重分析的纳米颗粒表征证实了该平台已成功开发出来。超声（US）辐射时说明的增强ROS时所示的活性氧（ROS）生成评估，亚甲基蓝降解和电子自旋共振测定法可以由美国激活的PPIX和低浓度H2O2的铁介导的芬顿反应产生（0.01％）。通过生长曲线和菌落形成单位测量值证明了体外抗肠球菌的粪便功效。共聚焦激光扫描显微镜和扫描电子显微镜观测结果说明了平台在根管中消除原位生物膜的有效性。由于ROS的氧化能力和短寿命的较强，Fenton反应增强的SDT可以在激活美国激活时诱导细菌的有害氧化损害，同时避免对细胞的非特异性毒性，通过使用CCK-8分析和形式的MC3T3-E1细胞通过细胞毒性评估来验证细胞毒性。与常用的NACLO相比，这种纳米板显示出理想的抗细菌，抗生物胶片能力和更好的生物相容性。这些结果表明，集成的M@P-FE + US + H2O2平台是美国增强根管灌溉和消毒的有前途的候选人。
The validity and biocompatibility of irrigating agents are imperative for the success of root canal therapy. The imperfections in the currently available irrigants highlight the fact that more advanced technologies and strategies are required for complete disinfection in endodontic treatments. In the present study, a Fenton reaction-enhanced antimicrobial sonodynamic therapy (SDT) platform was fabricated for root canal disinfection. Firstly, mesoporous silica nanoparticles (MSNs) were synthesized, grafted with an amino group and then conjugated with sonosensitizer protoporphyrin IX (PpIX). Iron ions were then anchored (M@P-Fe) to initiate a Fenton reaction. Nanoparticle characterization by size and zeta potential measurements, scanning electron microscopy, transmission electron microscopy and thermogravimetric analysis confirmed that the platform was successfully developed. Reactive oxygen species (ROS) generation assessment, methylene blue degradation and electron spin resonance assays illustrated upon ultrasound (US) irradiation, that augmented ROS, can be produced by US activated PpIX and iron mediated Fenton reactions from low concentration H2O2 (0.01%). In vitro anti-Enterococcus faecalis efficacy was demonstrated by growth curve and colony forming unit measurements. Confocal laser scanning microscopy and scanning electron microscopy observations illustrated the effectiveness of the platform on in situ biofilm eradication in root canal. Owing to the stronger oxidizing capability and short lifetime of ROS, the Fenton reaction-enhanced SDT can induce detrimental oxidative damage to bacteria upon activation of US while avoiding nonspecific toxicity to cells, which was verified by cytotoxicity evaluations using CCK-8 assay and morphology observation of MC3T3-E1 cells. Compared to commonly used NaClO, this nanoplatform displayed desirable anti-bacterial, anti-biofilm abilities and better biocompatibility. These results highlight that the integrated M@P-Fe + US + H2O2 platform is a promising candidate for US enhanced root canal irrigation and disinfection.