A population of neurons that produce hugin and express the diuretic hormone 44 receptor gene projects to the corpora allata in Drosophila melanogaster
A population of neurons that produce hugin and express the diuretic hormone 44 receptor gene projects to the corpora allata in Drosophila melanogaster
复制标题
DOI：
10.1111/dgd.12733
复制
发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Yosuke Mizuno;E. Imura;Yoshitomo Kurogi;Yuko Shimadaigu-Niwa;S. Kondo;Hiromu Tanimoto;Sebastian Hückesfeld;M. Pankratz;R. Niwa
中科院分区：
文献类型：
--
作者：
Yosuke Mizuno;E. Imura;Yoshitomo Kurogi;Yuko Shimadaigu-Niwa;S. Kondo;Hiromu Tanimoto;Sebastian Hückesfeld;M. Pankratz;R. Niwa
咽侧体是昆虫体内合成和分泌保幼激素（JH）的重要内分泌器官，对昆虫的多种发育和生理活动具有重要的调节作用。以往的研究表明，在许多昆虫物种中，CA直接受神经元支配，这意味着神经支配对于响应内部生理和外部环境调节JH生物合成是重要的。虽然这对于模式生物黑腹果蝇也是如此，但在CA投射神经元中产生哪些神经递质还有待澄清。本研究对D.在黑腹动物中，我们的目的是证明产生神经肽hugin（脊椎动物神经介肽U的无脊椎对应物）的神经元的子集直接投射到成年CA。在位于CA上的hugin神经元轴突末端观察到突触囊泡标记物，其用新产生的JH生物合成酶JH酸O-甲基转移酶（JHAMT）的抗体进行免疫标记。我们还发现CA投射的hugin神经元可能表达编码利尿激素44（Dh44）的特异性受体的基因。此外，我们的数据表明，CA投射hugin神经元与上游神经元产生Dh44的突触连接。据我们所知，这是第一次在D.黑腹，并解剖学特征的CA投射神经元和它们的上游神经元的神经通路。
The corpora allata (CA) are essential endocrine organs that biosynthesize and secrete the sesquiterpenoid hormone, namely juvenile hormone (JH), to regulate a wide variety of developmental and physiological events in insects. Previous studies had demonstrated that the CA are directly innervated with neurons in many insect species, implying the innervations to be important for regulating JH biosynthesis in response to internal physiology and external environments. While this is also true for the model organism, Drosophila melanogaster, which neurotransmitters are produced in the CA-projecting neurons are yet to be clarified. In this study on D. melanogaster, we aimed to demonstrate that a subset of neurons producing the neuropeptide hugin, the invertebrate counterpart of the vertebrate neuromedin U, directly projects to the adult CA. A synaptic vesicle marker in the hugin neurons was observed at their axon termini located on the CA, which were immunolabeled with a newly-generated antibody to the JH biosynthesis enzyme JH acid O-methyltransferase (JHAMT). We also found the CA-projecting hugin neurons to likely express a gene encoding the specific receptor for diuretic hormone 44 (Dh44). Moreover, our data suggested that the CA-projecting hugin neurons have synaptic connections with the upstream neurons producing Dh44. To the best of our knowledge, this is the first study to identify a specific neurotransmitter of the CA-projecting neurons in D. melanogaster, and to anatomically characterize a neuronal pathway of the CA-projecting neurons and their upstream neurons.