Correlation between miR-23a and onset of hepatocellular carcinoma
Correlation between miR-23a and onset of hepatocellular carcinoma
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DOI：
10.1016/j.clinre.2013.12.002
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发表时间：
2014-06-01
影响因子：
2.7
通讯作者：
Ren, Xianhua
中科院分区：
文献类型：
--
作者：
Bao, Lidao;Zhao, Jianfen;Ren, Xianhua
背景和目标：从细胞、基因和分子水平阐明miR-23 a在肝癌发生发展中的作用。分析肿瘤与癌旁组织的临床病理因素之间的关系。对影响总生存率和无复发生存率的危险因素进行多因素分析。结果：miR-23 a表达与TNM分期相关性最高，其次为肿瘤大小（P=0.041和0.047）。高miR-23 a、血管浸润、肿瘤大小≥ 7 cm、肿瘤包膜、病理分期较晚是影响总生存率的危险因素，影响无复发生存率的危险因素还包括甲胎蛋白水平≥ 200 μ g/L、肿瘤多发。与正常肝细胞株L-02相比，肿瘤细胞株SMMC-7721和HepG 2中miR-23 a的表达水平分别上调和下调。转染miR-23 a抑制剂抑制细胞生长。结论：miR-23 a高表达是影响总生存率和无复发生存率的独立预后因素，miR-23 a可能作为癌基因参与肝癌的发生。(C)2013年Elsevier Masson SAS。All rights reserved.
Background and aims: To clarify the role of miR-23a in the onset and development of hepatocarcinoma on the cellular, genetic and molecular levels.Patients and methods: Seventy-eight patients were included after hepatectomy. Relationships between the clinical pathological factors of tumor and paracancerous tissues were analyzed. Risk factors of overall and recurrence-free survival rates were subject to multi-variable analysis. Tissues were sequenced by digital miRNA expression profiling, and new miRNA was subject to target gene prediction.Results: miR-23a expression was correlated with the stage of the TNM Classification of Malignant Tumours most significantly, followed by tumor size (P=0.041 and 0.047). High miR-23a, vascular invasion, tumor size >= 7 cm, tumor capsule and late pathological stage were the risk factors of overall survival rate, and those of recurrence-free survival rate also included alpha-fetoprotein level >= 200 mu g/L and multiple tumors. Compared with normal hepatic cell line L-02, the miR-23a expression levels in tumor cell lines SMMC-7721 and HepG2 were up-regulated and down-regulated respectively. Transfecting miR-23a inhibitor suppressed cell growth. Apoptotic rates of the control and those transfected with inhibitor-NC and miR-23a inhibitor for 48 h were similar.Conclusion: High miR-23a expression is the independent prognostic factor of overall and recurrence free survival rates, and miR-23a may be involved in the onset of hepatocarcinoma as an oncogene. (C) 2013 Elsevier Masson SAS. All rights reserved.