Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway.
Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway.
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DOI：
10.3390/ijms18091948
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发表时间：
2017-09-11
影响因子：
5.6
通讯作者：
Furue M
中科院分区：
文献类型：
--
作者：
Hirano A;Goto M;Mitsui T;Hashimoto-Hachiya A;Tsuji G;Furue M
日本艾蒿，Artemisia princeps（日本yomogi），具有抗炎和抗氧化作用。已知含有茵陈蒿提取物（APE）的护肤产品可改善特应性皮炎中的皮肤干燥症状。特应性干燥皮肤与皮肤屏障蛋白如聚丝蛋白（FLG）和兜甲蛋白（LOR）的显著减少有关。最近，芳香烃受体（AHR）及其下游转录因子OVO-like 1（OVOL 1）已被证明可调节FLG和LOR的基因表达。本文的重点是评估APE对AHR/OVOL 1/FLG或LOR通路的影响，因为它们至今仍不清楚。我们首先证明，非细胞毒性浓度的APE显着上调抗氧化酶，NAD（P）H脱氢酶醌1和血红素加氧酶1，在人类角质形成细胞。即使在这些低浓度下，APE诱导AHR核转位，并显著上调CYP 1A 1（AHR激活的特异性靶基因）、FLG和LOR表达。AHR敲除下调OVOL 1表达。APE诱导的FLG和LOR上调在AHR或OVOL 1敲低的角质形成细胞中被取消。总之，抗氧化剂APE是一种有效的植物提取物，以AHR/OVOL 1依赖的方式上调FLG和LOR表达，这可能支持APE在治疗特应性干燥皮肤中的屏障修复作用。
The Japanese mugwort, Artemisia princeps (yomogi in Japanese), has anti-inflammatory and antioxidant effects. Skin care products containing Artemisia princeps extract (APE) are known to improve dry skin symptoms in atopic dermatitis. Atopic dry skin is associated with a marked reduction of skin barrier proteins, such as filaggrin (FLG) and loricrin (LOR). Recently, aryl hydrocarbon receptor (AHR), and its downstream transcription factor OVO-like 1 (OVOL1), have been shown to regulate the gene expression of FLG and LOR. The focus of this paper is to evaluate the effects of APE on the AHR/OVOL1/FLG or LOR pathway since they have remained unknown to this point. We first demonstrated that non-cytotoxic concentrations of APE significantly upregulated antioxidant enzymes, NAD(P)H dehydrogenase quinone 1 and heme oxygenase 1, in human keratinocytes. Even at these low concentrations, APE induced nuclear translocation of AHR and significantly upregulated CYP1A1 (a specific target gene for AHR activation), FLG, and LOR expression. AHR knockdown downregulated OVOL1 expression. The APE-induced upregulation of FLG and LOR was canceled in keratinocytes with AHR or OVOL1 knockdown. In conclusion, antioxidant APE is a potent phytoextract that upregulates FLG and LOR expression in an AHR/OVOL1-dependent manner and this may underpin the barrier-repairing effects of APE in treating atopic dry skin.
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