Interaction between host gastric sialyl-Lewis X and H-Pylori SabA enhances H-Pylori density in patients lacking gastric Lewis B antigen
Interaction between host gastric sialyl-Lewis X and H-Pylori SabA enhances H-Pylori density in patients lacking gastric Lewis B antigen
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DOI：
10.1111/j.1572-0241.2006.00358.x
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发表时间：
2006-01-01
影响因子：
9.8
通讯作者：
Wu, JJ
中科院分区：
文献类型：
--
作者：
Sheu, BS;Odenbreit, S;Wu, JJ
结果：我们检测了宿主胃Le（x）抗原与H. pylori感染测定胃内定植密度。评估幽门螺杆菌感染患者的细菌密度和胃Le（B）和唾液酸Le（x）表达。其对应的H. pylori分离株用PCR检测babA 2和sabA基因型。对sabA基因阳性PCR产物进行测序，以检查影响SabA表达的突变。结果：145株H. pylori分离株为BabA 2基因阳性，表达BabA。有116（80%）的sabA基因阳性的菌株，但只有45（31%）的菌株表达的SabA。对92株sabA基因阳性的PCR产物进行了测序，其中60%具有规则的CT重复对，另外40%具有独特的CT重复缺失。无论是删除，也不是不同的CT重复对在sabA区域是完全相关的SabA的表达，通过蛋白质印迹。H. pylori密度在表达胃唾液酸-Le（x）抗原（与SabA相互作用）的患者中较高（p < 0.001），仅在表达弱或无胃Le（B）抗原（与BabA相互作用）的患者中较高，而在胃Le（B）抗原明显表达的患者中不较高。pylori分离株100%为BabA阳性，但只有31%表达SabA。胃唾液酸Le（x）与H.幽门决定了弱表达或完全不表达胃Le（B）的患者的定植密度。
OBJECTIVES: We tested whether the interaction between host gastric Le(x) antigen and the SabA protein of H. pylori determined gastric colonization density.METHODS: A total of 145 H. pylori-infected patients were assessed for their bacterial density and gastric Le(b) and sialyl-Le(x) expression. Their corresponding H. pylori isolates were tested for babA2 and sabA genotype by PCR. The sabA-genopositive PCR products were sequenced to check for mutations affecting SabA expression. The BabA and SabA expressions of each isolate were confirmed by Western blotting.RESULTS: All 145 H. pylori isolates were babA2-genopositive and expressed BabA. There were 116 (80%) sabA-genopositive isolates, but only 45 (31%) of the isolates expressed SabA. Sequence of sabA-genopositive PCR products was achieved in 92 isolates, of which 60% had regular CT repeat-pairs and the other 40% had a unique deletion of the CT repeats. Neither the deletion nor the different CT repeat-pairs in the sabA region were totally correlated with SabA expression, defined by Western blotting. H. pylori density was higher in those expressing gastric sialyl-Le(x) antigen (which interacts with SabA) (p < 0.001) only in those patients expressing weak or no gastric Le(b) antigen (which would interact with BabA), not in those with evident expression of gastric Le(b) antigen.CONCLUSIONS: In Taiwan, H. pylori isolates are 100% BabA-positive, but only 31% of them express SabA. The interaction between gastric sialyl-Le(x) and SabA of H. pylori determines the colonization density of patients expressing gastric Le(b) weakly or not at all.