The Molecular Cloning of Platelet-Derived Growth Factor-C (PDGF-C) Gene of Gekko japonicus and Its Expression Change in the Spinal Cord After Tail Amputation
The Molecular Cloning of Platelet-Derived Growth Factor-C (PDGF-C) Gene of Gekko japonicus and Its Expression Change in the Spinal Cord After Tail Amputation
复制标题
壁虎血小板源性生长因子-C（PDGF-C）基因的分子克隆及其断尾后脊髓中的表达变化
DOI：
10.1007/s10571-008-9319-5
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Gu, Xiaosong
中科院分区：
文献类型：
--
作者：
Liu, Yan;Fan, Zheng;Gu, Xiaosong
从日本壁虎脑和脊髓的cDNA文库中获得了其血小板衍生生长因子-C(PDGF-C)基因。Northern杂交结果表明，壁虎PDGF-C基因转录本全长2.8kb，在心、肺、肾、卵巢等组织中大量表达。原位杂交显示脊髓灰质和白质均有阳性杂交信号。逆转录-聚合酶链式反应(RT-PCR)和Western印迹法检测断尾后脊髓组织中PDGF-C表达的变化。断尾后1d，脊髓组织中PDGF-C的表达水平最高，之后逐渐下降，直至2周，提示PDGF-C的表达水平可能与脊髓损伤和再生的过程有关。
The platelet-derived growth factor-C (PDGF-C) gene of Gekko japonicus was obtained from a brain and spinal cord cDNA library. The results of Northern blot showed that transcript of PDGF-C gene of gecko is 2.8 kb in length, and it was abundantly expressed in tissues of heart, lung, kidney, and ovary. In situ hybridization (ISH) revealed that positive hybridization signals were present in both gray matter and white matter of the spinal cord. The change of PDGF-C expression in the spinal cord after tail amputation was examined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The expression of PDGF-C in the spinal cord showed highest level at 1 day after tail amputation, and gradually decreased until 2 weeks, which indicated that the expression level of PDGF-C might be associated with the process of spinal cord injury and regeneration.