Polypyrimidine tract binding protein (hnRNP I) is possibly a conserved modulator of miRNA-mediated gene regulation.
Polypyrimidine tract binding protein (hnRNP I) is possibly a conserved modulator of miRNA-mediated gene regulation.
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DOI：
10.1371/journal.pone.0033144
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hutvagner G
中科院分区：
文献类型：
--
作者：
Engels B;Jannot G;Remenyi J;Simard MJ;Hutvagner G
MiRNA可以通过多种机制调节基因表达，导致靶mRNA表达的增加或减少，并且它可以影响特定细胞中数千种蛋白质的表达。越来越多的证据表明，miRNAs的作用可以通过与miRNAs靶向的相同3′ UTR结合的蛋白质来调节，这表明除了miRNAs及其靶位点之外的其他因素决定了基因表达的miRNAs调节。我们应用亲和纯化方案，使用生物素化的let-7 miRNA抑制剂分离参与let-7介导的基因调控的蛋白质，其导致聚嘧啶道结合蛋白（PTB）的亲和纯化。在这里，我们表明PTB与miRNA和人Argonaute 2（hAgo 2）通过RNA相互作用，以及鉴定出的由PTB和hAgo 2共同调控的潜在哺乳动物细胞靶点。此外，使用遗传学方法，我们已经证明了PTB与秀丽隐杆线虫let-7的遗传相互作用，表明PTB在miRNA介导的基因调控中的保守作用。
MiRNAs can regulate gene expression through versatile mechanisms that result in increased or decreased expression of the targeted mRNA and it could effect the expression of thousands of protein in a particular cell. An increasing body of evidence suggest that miRNAs action can be modulated by proteins that bind to the same 3′UTRs that are targeted by miRNAs, suggesting that other factors apart from miRNAs and their target sites determine miRNA-modulation of gene expression. We applied an affinity purification protocol using biotinylated let-7 miRNA inhibitor to isolate proteins that are involved in let-7 mediated gene regulation that resulted in an affinity purification of Polypyrimidine Tract Binding protein (PTB). Here we show that PTB interacts with miRNAs and human Argonaute 2 (hAgo2) through RNA as well as identified potential mammalian cellular targets that are co-regulated by PTB and hAgo2. In addition, using genetic approach, we have demonstrated that PTB genetically interacts with Caenorhabditis elegans let-7 indicating a conserved role for PTB in miRNA-mediated gene regulation.
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影响因子：
64.8
作者：
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通讯作者：
Bartel, David P.
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影响因子：
37.3
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
12.3
作者：
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通讯作者：
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影响因子：
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作者：
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