Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors.
Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors.
复制标题
DOI：
10.1182/blood.v86.7.2494.2494
复制
发表时间：
1995-10
期刊：
影响因子：
20.3
通讯作者：
M. Kobayashi;J. Laver;T. Kato;Hiroshi Miyazaki;M. Ogawa
中科院分区：
文献类型：
--
作者：
M. Kobayashi;J. Laver;T. Kato;Hiroshi Miyazaki;M. Ogawa
本文研究了重组人血小板生成素（TPO，Mpl配体）对体外人造血的影响。TPO单独不支持红细胞爆发形成，但在促红细胞生成素的存在下，它增强了从CD 34+骨髓和脐带血细胞的红细胞爆发形成。5%血清条件下的爆发促进活性（BPA）强于30%血清条件下。BPA效应的直接性质通过对早期红细胞爆发的重新接种研究得到证实。TPO的BPA低于白细胞介素-3，但与粒细胞/巨噬细胞集落刺激因子和钢因子相当。Mpl受体的可溶性形式抑制TPO的爆发增强效应，表明TPO的BPA效应是通过Mpl受体介导的。这些结果进一步描述了TPO的生理作用，并可能有助于确定TPO的临床用途。
We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst-promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony-stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.