Correlation of the neutral red uptake inhibition assay of cultured fathead minnow fish cells with fish lethality tests
Correlation of the neutral red uptake inhibition assay of cultured fathead minnow fish cells with fish lethality tests
复制标题
养殖黑头鲦鱼细胞中性红摄取抑制试验与鱼类致死试验的相关性
DOI：
10.1007/bf01689947
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发表时间：
1991
影响因子：
2.7
通讯作者：
Ingrid E. Vyver
中科院分区：
文献类型：
--
作者：
P. Dierickx;Ingrid E. Vyver
材料和方法SFHM细胞是已建立的鱼细胞系（美国典型培养物保藏中心NCC 142，得自Flow Laboratories，布鲁塞尔，比利时），来源于胖头鱼肛门后的组织。将细胞培养在添加10%胎牛血清、1%非必需氨基酸、100单位/mL青霉素和100 l的Dulbecco改良Eagle培养基（DMEM）中。tg/mL链霉素（完全培养基），并在34 ℃、5%CO2的潮湿气氛中孵育。中性红摄取抑制试验基本上按照Borenfreund和Shopsis（1985）的描述进行。用8通道移液管将细胞接种到滴定板（Nunc）的64个威尔斯孔中（每孔0.2 mL完全培养基中60，000个细胞）。在34 ° C下孵育24小时后，通过倒置多皿除去培养基。将培养物用0.2 mL等份的不同的
MATERIALS AND METHODSFHM cells are an established fish cell line (American Type Culture Collection N CC142, obtained from Flow Laboratories, Brussels, Belgium), derived from tissue posterior to the anus from fat head minnow. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum, 1% non-essential amino acids, 100 units/mL penicillin, and 100 l. tg/mL streptomycin (complete medium) and incubated at 34~ in a humidified atmosphere of 5% CO2.The neutral red uptake inhibition assay was essentially performed as described by Borenfreund and Shopsis (1985). The cells were seeded with an 8-channel pipet into 64 wells of a titer plate (Nunc)(60,000 cells in 0.2 mL complete medium per well). After incubation for 24 hr at 34~ the medium was removed by inverting the multidish. The cultures were treated with 0.2 mL aliquots of different