THE E7 PROTEINS OF THE NONONCOGENIC HUMAN PAPILLOMAVIRUS TYPE-6B (HPV-6B) AND OF THE ONCOGENIC HPV-16 DIFFER IN RETINOBLASTOMA PROTEIN-BINDING AND OTHER PROPERTIES
THE E7 PROTEINS OF THE NONONCOGENIC HUMAN PAPILLOMAVIRUS TYPE-6B (HPV-6B) AND OF THE ONCOGENIC HPV-16 DIFFER IN RETINOBLASTOMA PROTEIN-BINDING AND OTHER PROPERTIES
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DOI：
10.1128/jvi.64.2.723-730.1990
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发表时间：
1990-02-01
影响因子：
5.4
通讯作者：
WETTSTEIN, FO
中科院分区：
文献类型：
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作者：
GAGE, JR;MEYERS, C;WETTSTEIN, FO
致瘤性人乳头瘤病毒16型（HPV-16）的E7早期病毒蛋白与宫颈癌和癌源细胞系的恶性表型维持密切相关。hpv - 16e7是一种核磷酸化蛋白，它可以与ras合作转化幼鼠肾细胞，激活腺病毒E2启动子，并与视网膜母细胞瘤（RB）蛋白结合。非致癌性HPV-6b的E7磷酸化蛋白通常与良性生殖器疣有关，其氨基酸序列与HPV-16 E7相似，但在十二烷基硫酸钠-聚丙烯酰胺凝胶中的迁移、在非变性甘油梯度中的沉积以及与RB蛋白结合的能力方面存在显著差异。我们的研究结果表明，RB蛋白优先结合HPV-6b E7的磷酸化形式，这在瞬时转染的COS-7细胞中占E7总表达的一小部分。这些特征可能有助于解释致瘤型和非致瘤型生殖器乳头瘤病毒致瘤潜能的差异。
The E7 early viral protein of the oncogenic human papillomavirus type 16 (HPV-16) has been strongly implicated in the maintenance of the malignant phenotype in cervical cancers and cancer-derived cell lines. HPV-16 E7 is a nuclear phosphoprotein that can cooperate with ras to transform baby rat kidney cells, transactivates the adenovirus E2 promoter, and binds to the retinoblastoma (RB) protein. The E7 phosphoprotein of the nononcogenic HPV-6b, which is generally associated with benign genital warts, is similar to the HPV-16 E7 in amino acid sequence but differ dramatically in migration in sodium dodecyl sulfate-polyacrylamide gels, sedimentation in nondenaturing glycerol gradients, and the ability to bind the RB protein. Our results indicate that the RB protein preferentially binds the phosphorylated form of HPV-6b E7, which comprises a minor fraction of the total E7 expressed in transiently transfected COS-7 cells. These characteristics may help to explain the difference in the oncogenic potential of the oncogenic and nononcogenic types of genital papillomaviruses.