Identification of novel sRNAs involved in oxidative stress response in the fish pathogen Vibrio alginolyticus by transcriptome analysis
Identification of novel sRNAs involved in oxidative stress response in the fish pathogen Vibrio alginolyticus by transcriptome analysis
复制标题
通过转录组分析鉴定参与鱼类病原体溶藻弧菌氧化应激反应的新型 sRNA
DOI：
10.1111/jfd.12926
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发表时间：
2019
影响因子：
2.5
通讯作者：
Hu Zhong
中科院分区：
文献类型：
--
作者：
Peng Tao;Kan Jie;Lun Jingsheng;Hu Zhong
溶藻弧菌是水产养殖中的重要病原菌，在感染过程中会受到免疫系统产生的氧化应激。以往的研究表明sRNA在细菌的氧化应激反应中具有重要的功能，但在溶藻弧菌中发现的与氧化应激反应相关的sRNA较少。在这项研究中，通过RNA测序共鉴定了749种新的sRNA;其中，128种sRNA在氧化应激反应中上调或下调。此外，1，870个基因在氧化应激反应中表现出mRNA水平的变化。通过分析sRNA的靶基因，我们得出结论，这些sRNA可以调节负责铁转运、过氧化氢酶、GSH依赖的防御系统、电子传递和应激反应的基因的表达。此外，sRNA的功能也可能与溶藻弧菌的致病性有关。在此基础上，我们构建了溶藻弧菌氧化应激模型。本研究首次对sRNAs在溶藻弧菌氧化应激反应中的作用进行了展望。此外，这项研究可以帮助我们预防和控制水产养殖中这种重要的机会病原体。
Vibrio alginolyticusas an important pathogen in aquaculture can encounter the oxidative stress produced by the immune system during infection. Previous studies showed that sRNAs have important functions in response to oxidative stress in bacteria; however, less of sRNAs related to oxidative stress response were identified inV. alginolyticus. In this study, a total of 749 novel sRNAs were identified by RNA sequencing; among them, 128 sRNAs were up‐ or downregulated in response to oxidative stress. In addition, 1,870 genes exhibited variation on mRNA levels in oxidative stress response. By analysing the target genes of the sRNAs, we concluded that these sRNAs could regulate expressions of genes responsible for iron transport, catalase, GSH‐dependent defence system, electron transferred and stress response. Moreover, the functions of the sRNAs are also seemed related to the pathogenicity inV. alginolyticus. Based on the results, we constructed the oxidative stress model inV. alginolyticus. This study provides us the first outlook of sRNAs function in oxidative stress response inV. alginolyticus. Furthermore, this study can help us to prevent and control this important opportunistic pathogen in aquaculture.