Determination of Tyrosine and Tryptophan in Proteins
Determination of Tyrosine and Tryptophan in Proteins
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蛋白质中酪氨酸和色氨酸的测定
DOI：
10.1021/ac60128a025
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发表时间：
1957
影响因子：
7.4
通讯作者：
K. Schmid
中科院分区：
文献类型：
--
作者：
W. Bencze;K. Schmid
一种测定蛋白质中酪氨酸和色氨酸的新的分光光度法是基于在278和293 µ之间测量吸光度。与吸收曲线的两个特征最大值相切的直线的斜率指示这两种氨基酸的含量和比率。由于酪氨酸和色氨酸吸收光谱中的红移影响，在这些测定中引入的误差与以前的方法相比减小了。该方法还可用于某些蛋白质中氢键结合的非电离酪氨酸的测定。
A new spectrophotometric method for the determination of tyrosine and tryptophan in proteins is based upon measuring the absorbance in the range between 278 and 293 µ. The slope of the line drawn tangent to the two characteristic maxima of the absorption curve is indicative of the content and ratio of these two amino acids. The error introduced in these determinations by the influence of the bathochromic shift in the absorption spectra of tyrosine and tryptophan is diminished as compared with that of earlier methods. This method was further applied to the determination in certain proteins of the hydrogenbonded nonionizable tyrosine.