Complex regulation of the immunoglobulin mu heavy-chain gene enhancer: microB, a new determinant of enhancer function.
Complex regulation of the immunoglobulin mu heavy-chain gene enhancer: microB, a new determinant of enhancer function.
复制标题
免疫球蛋白 mu 重链基因增强子的复杂调控：microB，增强子功能的新决定因素。
DOI：
10.1128/mcb.10.6.3145-3154.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Sen,R
中科院分区：
文献类型：
--
作者：
Nelsen,B;Kadesch,T;Sen,R
免疫球蛋白μ重链基因增强子的B淋巴细胞特异性活性归因于增强子内存在的八聚体基序（ATTTGCAT），其结合称为NF-A2/OTF-2的B细胞特异性因子。然而，即使在删除该元件后，显著的残留增强子活性也表明存在第二个关键功能决定簇。我们已经使用删除和突变分析，以确定一个元素，PB（TTTGGGGAA），这是必不可少的B细胞特异性增强子活性在S194骨髓瘤细胞中的八聚体的情况下。在一组淋巴样细胞系中的转染分析表明，pB或八聚体的存在导致代表B细胞分化后期的细胞系中相当大的增强子活性，而这两种元素都是代表早期阶段的细胞系中所需的功能。此外，与前B细胞系中的结果相反，pB和八聚体元件在其中p增强子有活性的某些T细胞系中独立地起作用。
The B-lymphocyte-specific activity of the immunoglobulin μ heavy-chain gene enhancer has been attributed to the octamer motif (ATTTGCAT) present within the enhancer that binds a B-cell-specific factor designated NF-A2/OTF-2. However, significant residual enhancer activity even after deletion of this element has suggested the presence of a second critical functional determinant. We have used deletion and mutational analyses to define an element, pB (TTTGGGGAA), that is essential for B-cell-specific enhancer activity in S194 myeloma cells in the absence of the octamer. Transfection analysis in a panel of lymphoid cell lines suggests that the presence of either pB or octamer leads to considerable enhancer activity in cell lines representing later stages of B-cell differentiation, whereas both elements are needed for function in cell lines representing earlier stages. Furthermore, in contrast to the results in pre-B-cell lines, both pB and octamer elements function independently in certain T-cell lines in which the p enhancer is active.