Carotenoid composition and sequestration in cassava ( Manihot esculentum Crantz) roots
Carotenoid composition and sequestration in cassava ( Manihot esculentum Crantz) roots
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木薯 (Manihot esculentum Crantz) 根中的类胡萝卜素组成和封存
DOI：
10.1101/2023.12.19.572370
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Drapal M
中科院分区：
文献类型：
--
作者：
Drapal M
木薯（Manihot esculentum Crantz）是许多发展中国家的主食来源。它的可食用根富含淀粉，但缺乏微量营养素，如β-胡萝卜素。在目前的研究中，系谱育种群体的分析，导致鉴定木薯加入增强β-胡萝卜素含量高达40 μg/g DW。这相当于维生素A每日推荐摄入量（RDA）的0.2%。类胡萝卜素途径的β-分支在木薯根中占主导地位，β-胡萝卜素占主导地位，其次是β-环衍生的类胡萝卜素的其他次要环氧化物。代谢组学分析表明，中间代谢的稳态水平没有改变类胡萝卜素的形成，类似于淀粉和碳水化合物的水平。脱辅基类胡萝卜素似乎是独立的类胡萝卜素前体。脂质组学分析提供了证据，类胡萝卜素和脂质含量之间的显着正相关，特别是质体特异性半乳糖脂。分离的造粉体的蛋白质组学分析鉴定了大多数与翻译和碳水化合物/淀粉生物合成相关的蛋白质（例如葡萄糖-1-磷酸腺苷酰转移酶）。即使在类胡萝卜素含量最高的品系中也没有检测到类胡萝卜素相关蛋白。类胡萝卜素与通常注释为质体膜和质体膜（特别是包膜）的组分相关。蛋白质组学分析证实了这些结构除了plastapuli，因此潜在的淀粉体结构可能不包含经典的plastapuli结构。
Cassava (Manihot esculentum Crantz) is a staple food source for many developing countries. Its edible roots are high in starch but lack micronutrients such as β-carotene. In the present study, analysis of pedigree breeding populations has led to the identification of cassava accessions with enhanced β-carotene contents up to 40 μg/g DW. This represents 0.2% of the Recommended Daily Allowance (RDA) for vitamin A. The β-branch of the carotenoid pathway predominates in cassava roots, with dominant levels of β-carotene followed by other minor epoxides of β-ring derived carotenoids. Metabolomic analysis revealed that steady state levels of intermediary metabolism were not altered by the formation of carotenoids, similar to starch and carbohydrate levels. Apocarotenoids appeared to be independent of their carotenoid precursors. Lipidomic analysis provided evidence of a significant positive correlation between carotenoid and lipid content, in particular plastid specific galactolipids. Proteomic analysis of isolated amyloplasts identified the majority of proteins associated with translation and carbohydrate/starch biosynthesis (e.g. glucose-1-phosphate adenylyltransferase). No carotenoid related proteins were detected even in the highest carotenoid containing lines. Carotenoids were associated with fractions typically annotated as plastoglobuli and plastid membranes (particularly the envelope). Proteomic analysis confirmed these structures apart from plastoglobuli, thus potentially amyloplast structures may not contain classical plastoglobuli structures.