Oral Administration of Geranylgeraniol Rescues Denervation-induced Muscle Atrophy via Suppression of Atrogin-1
Oral Administration of Geranylgeraniol Rescues Denervation-induced Muscle Atrophy via Suppression of Atrogin-1
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DOI：
10.21873/invivo.12047
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发表时间：
2020-09-01
期刊：
影响因子：
2.3
通讯作者：
Kokabu, Shoichiro
中科院分区：
文献类型：
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作者：
Miyawaki, Aki;Rojasawasthien, Thira;Kokabu, Shoichiro
背景/目的：香叶基香叶醇（GGOH），一种C20类异戊二烯天然存在于几种食物中。我们先前报道了GGOH处理降低了Atrogin-1的表达水平，Atrogin-1参与骨骼肌降解并刺激C2 C12成肌细胞的肌源性分化。然而，GGOH补充对体内骨骼肌代谢的影响尚不清楚。材料与方法：采用去神经支配的方法复制骨骼肌萎缩模型。Atrogin-1的表达水平通过蛋白质印迹或真实的时间PCR来评估。结果如下：口内给药GGOH减少了肌纤维横截面积的减少，也抑制了Atrogin-1在去神经诱导的肌肉萎缩中的表达水平。此外，GGOH治疗抑制Atrogin-1的表达和糖皮质激素在体外骨骼肌纤维尺寸的减少。结论：口内给予GGOH通过抑制Atrogin-1来挽救失神经诱导的肌肉萎缩。
Background/Aim: Geranylgeraniol (GGOH), a C20 isoprenoid naturally occurs in several foods. We previously reported that GGOH treatment reduced the expression levels of Atrogin-1 which is involved in skeletal muscle degradation and stimulates the myogenic differentiation of C2C12 myoblasts. However, the effect of GGOH supplementation on skeletal muscle metabolism in vivo is unknown. Materials and Methods: Skeletal muscle atrophy was induced by denervation. The expression levels of Atrogin-1 were assessed by western blotting or real time PCR. Results: Intraoral administration of GGOH reduced the decrease in the cross-sectional area of muscle fibers and also suppressed the expression levels of Atrogin-1 in denervation induced muscle atrophy. Also, GGOH treatment suppressed the expression of Atrogin-1 and the decrease in skeletal muscle fiber size by glucocorticoid in vitro. Conclusion: Intraoral administration of GGOH rescues denervation-induced muscle atrophy via suppression of Atrogin-1.