Corneal epithelial cell growth over tethered-protein/peptide surface-modified hydrogels.
Corneal epithelial cell growth over tethered-protein/peptide surface-modified hydrogels.
复制标题
角膜上皮细胞在束缚蛋白/肽表面修饰的水凝胶上生长。
DOI：
10.1002/jbm.b.30131
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
Gebhardt,BryanM
中科院分区：
文献类型：
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作者：
Jacob,JeanT;Rochefort,JamesR;Bi,Jingjing;Gebhardt,BryanM
在这项研究中，我们研究了(1)细胞外基质蛋白[纤维连接蛋白、层粘连蛋白、P物质和胰岛素样生长因子-1(IGF-1)]和(2)多肽序列[RGD和纤维连接蛋白黏附促进肽(FAP)]在聚乙二醇链上连接到新型水凝胶上的角膜上皮细胞的生长速度和黏附能力。比较了普通聚甲基丙烯酸-甲基丙烯酸羟乙酯(PHEMA/MAA)水凝胶、包被细胞外基质蛋白或多肽的PHEMA/MAA水凝胶和表面带有细胞外基质蛋白或多肽的PHEMA/MAA水凝胶对原代兔角膜上皮细胞融合生长的影响。F-肌动蛋白染色检测生长于表面的上皮细胞的灶性粘连情况。在15天的培养期间，平坦的水凝胶表面几乎没有上皮细胞生长。在涂层水凝胶中，只有纤维连接蛋白涂层的表面与普通水凝胶相比，细胞生长显著增加(p<；0.009)。然而，即使是这些表面也只达到了最大20%的融合。层粘连蛋白、纤维连接蛋白黏附促进肽(FAP)和纤维连接蛋白/层粘连蛋白(1：1)系绳修饰水凝胶在培养结束时均达到100%融合，尽管达到融合的比率不同。F-肌动蛋白染色显示，层粘连蛋白、FAP和纤维连接蛋白/层粘连蛋白系绳修饰的表面形成局灶性粘连。这些结果支持这样的假设，即与蛋白涂层或普通水凝胶表面相比，将某些细胞外基质蛋白和/或肽拴在水凝胶表面可以增强上皮细胞的生长和黏附。#2004威利期刊公司生物材料资源B部分：应用生物材料72B：198-205,2005
In this study, we investigated the corneal epithelial cell growth rate and adhesion to novel hydrogels with (1) extracellular matrix proteins [fibronectin, laminin, substance P, and insulin‐like growth factor‐1 (IGF‐1)] and (2) peptide sequences [RGD and fibronectin adhesion‐promoting peptide (FAP)] tethered to their surface on poly(ethylene glycol) (PEG) chains. The growth rate to confluence of primary rabbit cornea epithelial cells was compared for plain polymethacrylic acid‐co‐hydroxyethyl methacrylate (PHEMA/MAA) hydrogels, PHEMA/MAA hydrogels coated with extracellular matrix proteins or peptides, and PHEMA/MAA hydrogels with tethered extracellular matrix proteins or peptides on the surface. The development of focal adhesions by the epithelial cells grown on the surfaces was determined by F‐actin staining. Little to no epithelial cell growth occurred on the plain hydrogel surfaces throughout the 15‐day culture period. Of the coated hydrogels, only the fibronectin‐coated surfaces showed a significant increase in cell growth compared to plain hydrogels (p< 0.009). However, even these surfaces reached a maximum of only 20% confluence. Laminin, fibronectin adhesion‐promoting peptide (FAP), and fibronectin/laminin (1:1) tether‐modified hydrogels all achieved 100% confluence by the end of the culture period, although the rates at which confluence was reached differed. F‐actin staining showed that focal adhesions were formed for the laminin, FAP, and fibronectin/laminin tether‐modified surfaces. The results support the hypothesis that tethering certain extracellular matrix proteins and/or peptides to the hydrogel surface enhances epithelial cell growth and adhesion, compared with that seen for protein‐coated or plain hydrogel surfaces. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 198–205, 2005