Sterol 14-demethylase P450 activity expressed in rat gonads: contribution to the formation of mammalian meiosis-activating sterol.
Sterol 14-demethylase P450 activity expressed in rat gonads: contribution to the formation of mammalian meiosis-activating sterol.
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DOI：
10.1006/bbrc.1996.0929
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发表时间：
1996-06
影响因子：
3.1
通讯作者：
Y. Yoshida;C. Yamashita;M. Noshiro;M. Fukuda;Y. Aoyama
中科院分区：
文献类型：
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作者：
Y. Yoshida;C. Yamashita;M. Noshiro;M. Fukuda;Y. Aoyama
4，4-二甲基胆甾醇-8，14，24-三烯醇的形成依赖于固醇14-脱甲基酶P450(P45014 DM)，已被报道为哺乳动物减数分裂激活的甾醇。观察到卵巢P45014-DM活性的一过性升高，可能与雌性大鼠的性周期有关。孕马血清促性腺激素可诱导未成熟卵巢P45014 DM活性。这些事实表明，卵巢P45014 DM参与了促性腺激素依赖的减数分裂激活类固醇的形成，并为理解促性腺激素启动哺乳动物卵母细胞减数分裂恢复的机制提供了线索。还发现大鼠睾丸P45014 DM参与了4，4-二甲基-8，24-二烯醇的产生，这是从公牛睾丸中分离到的另一种减数分裂激活的甾醇。
Sterol 14-demethylase P450 (P45014DM)-dependent formation of 4,4-dimethylcholesta-8,14,24-trienol, that has been reported for a mammalian meiosis-activating sterol, in rat ovaries was confirmed. Transient elevation of ovarian P45014DM activity, that might be related to the female sexual cycle of rat, was observed. The P45014DM activity of immature ovaries was induced by pregnant mare's serum gonadotropin. These facts suggest the contribution of ovarian P45014DM to the gonadotropin-dependent formation of the meiosis-activating sterol, and provide a clue to understanding the mechanism how gonadotropins initiate the resumption of meiosis of mammalian oocytes. It was also found that rat testicular P45014DM participated in the production of 4,4-dimethylcholesta-8,24-dienol that was reported for another meiosis-activated sterol isolated from bull testes.