Geographic discrimination of Paracoccidioides brasiliensis strains by randomly amplified polymorphic DNA analysis
Geographic discrimination of Paracoccidioides brasiliensis strains by randomly amplified polymorphic DNA analysis
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DOI：
10.1128/jcm.36.6.1733-1736.1998
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发表时间：
1998-06-01
影响因子：
9.4
通讯作者：
San-Blas, G
中科院分区：
文献类型：
--
作者：
Calcagno, AM;Niño-Vega, G;San-Blas, G
随机扩增多态性DNA（RAPD）分析33 Paracoccidioides brasiliensis菌株从阿根廷，巴西，哥伦比亚，秘鲁，委内瑞拉产生可重复的扩增产物，这是足够的多态性，让菌株的分化。用5个引物（OPG 03、OPG 05、OPG 14、OPG 16和OPG 18）产生的分型结果具有较高的区分指数（0.956），而仅用2个引物（OPG 3和OPG 14）产生的分型区分指数略有降低（0.940）。基于这些结果的树状图显示菌株之间的高度相似性，并且遗传差异在与地理区域相关的簇中表达，但与疾病的病理特征无关。除了少数例外，菌株按地理来源分为如下五组：组I，委内瑞拉菌株;组II，巴西菌株;组III，秘鲁菌株;组IV，哥伦比亚菌株;组IV，巴西菌株。V组为阿根廷株。含有最不同菌株的组是组V（判别指数，0.633）;其它四组的判别指数为0.824。引物OPG 18本身的使用足以判别种特异性，引物OPG 14本身的使用足以判别样品中菌株的地理位置。该方法可为巴西对虾的流行病学研究提供参考。
Randomly amplified polymorphic DNA (RAPD) analysis of 33 Paracoccidioides brasiliensis strains from Argentina, Brazil, Colombia, Peru, and Venezuela produced reproducible amplification products which were sufficiently polymorphic to allow differentiation of the strains. Types generated with five primers (OPG 03, OPG 05, OPG 14, OPG 16, and OPG 18) resulted in a high discriminatory index (0.956), The discriminatory index was slightly reduced (0.940) when only two primers (OPG 3 and OPG 14) were used. A dendrogram based on these results showed a high degree of similarity among the strains, and genetic differences were expressed in clusters related to geographical regions but not to pathological features of the disease, With a few exceptions, strains were sorted into five groups by geographical origin as follows: group I, Venezuelan strains; group II, Brazilian strains; group III, Peruvian strains; group TV, Colombian strains; and group V, Argentinian strains. The group containing the most disparate strains was group V (discriminatory index, 0.633); the discriminatory index for the other four groups was 0.824, The use of primer OPG 18 by itself was sufficient to discriminate species specificity, and the use of primer OPG 14 by itself was sufficient to discriminate among the geographical locations of the strains in the sample. This method may be helpful for epidemiological studies of P. brasiliensis.