The in vitro effects of dehydroepiandrosterone on chondrocyte metabolism.
The in vitro effects of dehydroepiandrosterone on chondrocyte metabolism.
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DOI：
10.1016/j.joca.2005.09.012
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发表时间：
2006-03
影响因子：
7
通讯作者：
J-S Sun;C.-X. Wu;Y. Tsuang;L-T Chen;S. Sheu
中科院分区：
文献类型：
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作者：
J-S Sun;C.-X. Wu;Y. Tsuang;L-T Chen;S. Sheu
目的探讨脱氢表雄酮（DHEA）对体外培养的新生大鼠软骨细胞的作用。方法采用三维琼脂糖微球培养新生大鼠软骨细胞，并经DHEA处理。通过分析软骨细胞增殖、基质蛋白合成、胶原、基质金属蛋白酶-1、-3和-13基因表达来评价DHEA对软骨细胞活性的影响（MMP-1、-3和-13）和环氧合酶-2（COX-II），以及白细胞介素-6（IL-6）的蛋白质合成，前列腺素E_2（PGE_2）和金属蛋白酶组织抑制剂-1（TIMP-1）。DHEA抑制MMP-1的表达，脂多糖（LPS）可促进COX-2、COX-3、COX-13基因和PGE-2蛋白的合成，而抑制COX-2和iNOS基因的表达。结论DHEA能调节新生软骨细胞MMPs和PGE 2的失衡，提示DHEA对新生软骨细胞的损伤具有潜在的保护作用。软骨损伤
OBJECTIVETo investigate the in vitro effects of dehydroepiandrosterone (DHEA) on neonatal rat chondrocytes.DESIGNChondrocytes isolated from neonatal rat cartilage were cultured in three-dimensionally agarose beads and were treated with DHEA.METHODSPrimary culture of chondrocytes was harvested from newborn Wistar rats. The DHEA effects on chondrocyte activities were evaluated by analyzing chondrocyte proliferation, matrix protein synthesis, gene expressions of collagen, matrix metalloproteinase-1, -3 and -13 (MMP-1, -3 and -13), and cyclooxygenase-2 (COX-II), and protein synthesis of interleukin-6 (IL-6), prostaglandin E2 (PGE2) and tissue inhibitor of metalloproteinase-1 (TIMP-1).RESULTSThe DHEA treatment did affect chondrocyte proliferation and glycosaminoglycan (GAG) synthesis. DHEA suppressed the expression of MMP-1, -3 and -13 genes and PGE2 protein synthesis enhanced by lipopolysaccharide (LPS) while the COX-II and inducible nitric oxide synthase (iNOS) gene expressions were down-regulated by DHEA.CONCLUSIONSOur study demonstrates that DHEA has an ability to modulate the imbalance between MMPs and PGE2 in the neonatal chondrocytes which suggest that it has a potential protective role against articular cartilage damage.