Metabolic patterns in three types of phagocytizing cells.
Metabolic patterns in three types of phagocytizing cells.
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三种类型的吞噬细胞中的代谢模式。
DOI：
10.1083/jcb.17.3.487
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发表时间：
1963-06
影响因子：
7.8
通讯作者：
KARNOVSKY, M L
中科院分区：
文献类型：
--
作者：
OREN, R;FARNHAM, A E;SAITO, K;MILOFSKY, E;KARNOVSKY, M L
本文比较了豚鼠腹腔渗出液中多形核白细胞和单核细胞以及同一动物肺泡巨噬细胞的某些化学和代谢特性。在吞噬作用的过程中，这三种类型的细胞的代谢模式发生了变化。还测定了常规代谢抑制剂和厌氧作用对上述三种细胞类型中每一种的吞噬能力的影响。从这些研究中发现，肺泡巨噬细胞在相当大的程度上依赖于氧化磷酸化为吞噬作用提供能量。其他两种类型的细胞仅依赖于糖酵解作为该功能的代谢能量来源。在一些旨在获得信息的复杂的脂质在细胞膜的功能可能发挥的作用的实验中，有人指出，吞噬作用刺激无机磷酸盐-P32掺入两种类型的细胞从腹膜渗出液的磷脂，无论这些是自由游泳或粘附到表面。在肺泡巨噬细胞的情况下尚未检测到这种现象。
Some chemical and metabolic characteristics of polymorphonuclear leukocytes and monocytes from peritoneal exudates of the guinea pig, and of alveolar macrophages from the same animal, have been compared. Changes in the metabolic patterns of these three types of cell have been followed during the act of phagocytosis. The effect of conventional inhibitors of metabolism, and of anaerobiosis on the phagocytic ability of each of the three cell types mentioned has also been determined. From these studies it was found that alveolar macrophages depend to a considerable degree upon oxidative phosphorylation to provide energy for phagocytosis. The other two types of cell depend only on glycolysis as the source of metabolic energy for that function. In some experiments aimed at obtaining information on the possible role of complex lipids in the function of the cell membrane, it was noted that phagocytosis stimulated the incorporation of inorganic phosphate-P32 into the phosphatides of both types of cell from peritoneal exudates—whether these were free-swimming or adherent to a surface. This phenomenon has not yet been detected in the case of alveolar macrophages.