SCHISTOSOMA-MANSONI - EFFECT OF INFECTION ON REPRODUCTION AND GONADAL GROWTH IN BIOMPHALARIA-GLABRATA
SCHISTOSOMA-MANSONI - EFFECT OF INFECTION ON REPRODUCTION AND GONADAL GROWTH IN BIOMPHALARIA-GLABRATA
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DOI：
10.1016/0014-4894(89)90114-8
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发表时间：
1989-04-01
影响因子：
2.1
通讯作者：
YOSHINO, TP
中科院分区：
文献类型：
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作者：
CREWS, AE;YOSHINO, TP
将性成熟的光滑双脐螺暴露于12个曼氏血吸虫毛蚴中，并在5周内监测螺的产卵量。在研究期间，接触的蜗牛以与未接触的对照组大致相同的速度生长。阉割，作为衡量每只蜗牛产卵的平均数量减少，发生在14和21天postexhibition（PE）。感染钉螺的生殖力下降与子孢子囊在消化腺和性腺中的迁移和建立相一致。在卵睾丸纵切片中单个卵母细胞的计数显示，未感染的蜗牛在27，31和40天PE时每个切片中包含的卵母细胞显著多于感染的蜗牛。此外，在40天的实验期间，控制螺的性腺部分的平均面积增加，而感染螺的性腺面积没有这样的增加。这些数据表明，有一个在感染的蜗牛性腺生长抑制。当卵母细胞数据表示的平均性腺面积，未感染和感染的蜗牛每平方毫米的性腺的卵母细胞的平均数没有显着差异，在研究期间，除了在第14天PE，当感染的蜗牛包含一个显着更多的卵母细胞每平方毫米的性腺比未感染的控制。推测S.曼森氏菌主要负责抑制宿主的生殖活动，并可能通过参与调节性腺生长的机制介导其影响。然而，目前尚不清楚这种作用是直接介导的性腺或间接通过改变蜗牛宿主代谢。
Sexually mature Biomphalaria glabrata were exposed to 12 miracidia of Schistosoma mansoni, and egg production of snails was monitored over a period of 5 weeks. During the study period, exposed snails grew at approximately the same rate as unexposed controls. Castration, as measured by a reduction in the mean number of eggs laid per snail, occurred between 14 and 21 days postexposure (PE). The reduction in fecundity in infected snails coincided with the migration and establishment of daughter sporocysts in the digestive gland and gonad. Enumeration of individual oocytes in longitudinal sections of the ovotestis revealed that uninfected snails contained significantly more oocytes per section than infected snails at 27, 31, and 40 days PE. In addition, the mean area of gonadal sections of control snails increased over the 40-day experimental period, whereas there was no such increase in gonadal area of infected snails. These data suggest that there is an inhibition in gonadal growth in infected snails. When oocyte data were expressed in terms of mean gonadal area, the mean number of oocytes per mm2 of gonad of uninfected and infected snails did not differ significantly over the study period, except at Day 14 PE, when infected snails contained a significantly greater number of oocytes per mm2 of gonad than did uninfected controls. It is hypothesized that daughter sporocysts of S. mansoni are primarily responsible for the inhibition of host reproductive activity, and may be mediating their effects through mechanisms involved in the regulation of gonadal growth. However, it is not known whether such action is mediated directly on the gonads or indirectly through alterations in snail host metabolism.