Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula
Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula
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DOI：
10.1111/aos.12427
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发表时间：
2014-11-01
影响因子：
3.4
通讯作者：
Yamamoto, Naoki
中科院分区：
文献类型：
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作者：
Chong, Pei Pei;Tung, Chee Hong;Yamamoto, Naoki
目的本研究的目的是确定人乳头瘤病毒（HPV）在赤道马来半岛不同族群的原发性和复发性翼状胬肉样本中的患病率。方法从45例新鲜获得的原发和复发性翼状胬肉患者标本和11例无眼表病变的志愿者正常结膜拭子中提取dna。巢式PCR检测HPV DNA的存在。pcr阳性样本进行DNA测序以确定HPV基因型。采用HPV16和HPV18型特异性TaqMan探针实时荧光定量PCR检测病毒DNA拷贝数。结果在DNA质量合格的45份翼状胬肉标本中，HPV DNA阳性29份（64.4%），而正常结膜拭子均为HPV阴性。18型基因型最多（41.4%），其次是16型基因型（27.6%）。不常见的HPV58和HPV59各有一例。其中7个样本同时感染了HPV16和HPV18。所有四个已知的复发性翼状胬肉样本都是hpv阳性，而研究中唯一的早期翼状胬肉样本是hpv阴性。hpv阳性状态与性别或年龄没有显著关联。来自印度族裔群体的患者（6人中有5人）的HPV阳性比例很高，而马来患者的HPV阳性比例高于华人。HPV18样品的病毒载量在2 × 10(2) ~ 3 × 10(4) copies / g之间，而HPV16样品的病毒载量为4 × 10(1) ~ 10(2) copies / g。结论本文首次报道了翼状胬肉样品中HPV病毒DNA的定量测定。在我们的样本中，高患病率的致癌HPV提示了HPV在翼状胬肉发病机制中的可能作用。此外，相对较低的HPV病毒载量与这种眼部疾病的癌前性质一致。
PurposeThe aim of the study was to determine the prevalence of human papillomavirus (HPV) in primary and recurrent pterygia samples collected from different ethnic groups in the equatorial Malay Peninsula.MethodsDNA was extracted from 45 specimens of freshly obtained primary and recurrent pterygia from patients and from 11 normal conjunctival swabs from volunteers with no ocular surface lesion as control. The presence of HPV DNA was detected by nested PCR. PCR-positive samples were subjected to DNA sequencing to determine the HPV genotypes. Real-time PCR with HPV16 and HPV18 type-specific TaqMan probes was employed to determine the viral DNA copy number.ResultsOf 45 pterygia samples with acceptable DNA quality, 29 (64.4%) were positive for HPV DNA, whereas all the normal conjunctiva swabs were HPV negative. Type 18 was the most prevalent (41.4% of positive samples) genotype followed by type 16 (27.6%). There was one case each of the less common HPV58 and HPV59. Seven of the samples harboured mixed infections of both HPV16 and HPV18. All the four known recurrent pterygia samples were HPV-positive, whereas the sole early-stage pterygium sample in the study was HPV-negative. There was no significant association between HPV-positive status with gender or age. A high proportion of patients from the Indian ethnic group (five of six) were HPV-positive, whereas the Malay patients were found to have higher HPV positivity than the Chinese. The viral load of HPV18 samples ranged between 2 x 10(2) and 3 x 10(4) copies per g, whereas the viral load of HPV16 specimen was 4 x 10(1) to 10(2) copies per g.ConclusionThis report describes for the first time the quantitative measurement of HPV viral DNA for pterygium samples. The high prevalence of oncogenic HPVs in our samples suggests a possible role for HPV in the pathogenesis of pterygia. Moreover, the relatively low HPV viral load is concordant with the premalignant nature of this ocular condition.