Diversity of siderophore genes encoding biosynthesis of 2,3-dihydroxybenzoic acid in Aeromonas spp.
Diversity of siderophore genes encoding biosynthesis of 2,3-dihydroxybenzoic acid in Aeromonas spp.
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气单胞菌属中编码 2,3-二羟基苯甲酸生物合成的铁载体基因的多样性。
DOI：
10.1007/bf00149553
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Byers,BR
中科院分区：
文献类型：
--
作者：
Massad,G;Arceneaux,JE;Byers,BR
虽然有两种细菌产生肠杆菌素，肠杆菌素是许多肠道细菌的铁载体，但大多数空气单胞菌属产生单胞杆菌素中的铁载体。两种铁载体均含有2,3-二羟基苯甲酸（2,3- dhb）。从产生肠杆菌的气单胞杆菌分离株中克隆出与大肠杆菌2,3- dhb操纵子entCEBA（P15）的肠杆菌蛋白基因突变互补的铁载体基因（编号为aebc， -E， -带- a，用于气单胞杆菌的生物合成）。气单胞菌基因图谱显示，其基因序列为aebceba，与theE相同。coli2 3-DHB操纵子。空气单胞菌基因和foramoA（先前从产生单胞菌素的空气单胞菌中克隆的entc等效基因）的基因探针没有交叉杂交。基因探测theE。大肠杆菌2,3- dhb基因与空气单胞菌不杂交。DNA。因此，在气单胞菌中，2,3- dhb的合成由两个不同的基因群编码；一种（amo）存在于单肠杆菌素产生菌中，而另一种（aeb）存在于肠杆菌素产生菌中。这些系统中的每一个都不同于theE（但在功能上与之相关）。coli2 3-DHB操纵子。这些基因可能是从祖先的2,3- dhb基因群中分化出来的。
Most species of the genusAeromonasproduce the siderophore amonabactin, although two species produce enterobactin, the siderophore of many enteric bacteria. Both siderophores contain 2,3-dihydroxybenzoic acid (2,3-DHB). Siderophore genes (designatedaebC, -E, -Band-A, foraeromonadenterobactinbiosynthesis) that complemented mutations in the enterobactin genes of theEscherichia coli2,3-DHB operon,entCEBA(P15), were cloned from an enterobactin-producing isolate of theAeromonasspp. Mapping of the aeromonad genes suggested a gene order ofaebCEBA, identical to that of theE. coli2,3-DHB operon. Gene probes for the aeromonadaebCEgenes and foramoA(theentC-equivalent gene previously cloned from an amonabactin-producingAeromonasspp.) did not cross-hybridize. Gene probes for theE. coli2,3-DHB genesentCEBAdid not hybridize withAeromonasspp. DNA. Therefore, in the genusAeromonas, 2,3-DHB synthesis is encoded by two distinct gene groups; one (amo) is present in the amonabactin-producers, while the other (aeb) occurs in the enterobactin-producers. Each of these systems differs from (but is functionally related to) theE. coli2,3-DHB operon. These genes may have diverged from an ancestral group of 2,3-DHB genes.