Carbon monoxide-induced suspended animation protects against hypoxic damage in Caenorhabditis elegans
Carbon monoxide-induced suspended animation protects against hypoxic damage in Caenorhabditis elegans
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DOI：
10.1073/pnas.0403312101
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发表时间：
2004-06-15
影响因子：
11.1
通讯作者：
Roth, MB
中科院分区：
文献类型：
--
作者：
Nystul, TG;Roth, MB
缺氧是细胞损伤和死亡的主要原因。在这里，我们证明，秀丽隐杆线虫胚胎，可以生存在缺氧（< 0.001 kPa O-2）通过进入假死和轻度缺氧（0.25-1 kPa O-2）通过缺氧诱导因子1介导的反应，不能生存在中等浓度的氧气，0.01和0.1 kPa O-2。此外，我们表明，一氧化碳可以保护C。线虫胚胎在此敏感范围内抗缺氧损伤。一氧化碳也可以拯救缺氧敏感突变体hif-1（ia 04）免于缺氧致死。这项工作定义了缺氧损伤发生在C。elegans胚胎，并证明一氧化碳可以通过诱导假死来防止这种损害。
Oxygen deprivation is a major cause of cellular damage and death. Here we demonstrate that Caenorhabditis elegans embryos, which can survive both in anoxia (< 0.001 kPa O-2) by entering into suspended animation and in mild hypoxia (0.25-1 kPa O-2) through a hypoxia-inducible factor 1-mediated response, cannot survive in intermediate concentrations of oxygen, between 0.01 and 0.1 kPa O-2. Moreover, we show that carbon monoxide can protect C. elegans embryos against hypoxic damage in this sensitive range. Carbon monoxide can also rescue the hypoxia-sensitive mutant hif-1(ia04) from lethality in hypoxia. This work defines the oxygen tensions over which hypoxic damage occurs in C. elegans embryos and demonstrates that carbon monoxide can prevent this damage by inducing suspended animation.